基于细胞电阻抗传感器的细胞多生理参数分析系统设计  

Cellular Multiphysiological Parameter Analysis System Based on Electrical Cell-Substrate Impedance Sensor

作　　者：王天星[1] 黎洪波[1] 苏凯麒[1] 胡宁[1] 王平[1] 

机构地区：[1]浙江大学生物传感器国家专业实验室,生物医学工程教育部重点实验室,生仪学院,杭州310027

出　　处：《传感技术学报》2014年第12期1589-1595,共7页Chinese Journal of Sensors and Actuators

基　　金：海洋公益项目(201305010)

摘　　要：针对传统的细胞传感器系统存在参数单一的问题,设计了基于细胞电阻抗传感器的细胞多生理参数分析系统。通过阻抗传感器的模型及其生长和搏动原理、阻抗传感器的设计加工,细胞多生理参数分析系统设计等方面对系统进行介绍。该系统具有操作简便,高一致性和高通量等特点。采用系统测试实验和细胞实验对系统的基本性能继续测试。实验结果表明,细胞多生理参数分析系统能同时检测细胞生长和心肌细胞的搏动,具备快速,长期,无损和高通量测量的特点,为细胞生理研究提供了实用的平台。For solving problems of single parameters of traditional sensor system,Cellular multiphysiological parame-ter analysis system based on electrical cell-substrate impedance sensor（ ECIS） was designed. The equivalent model of ECIS,cellular growth and beating detection principle,the design and fabrication of ECIS,cellular multiphysiologi-cal parameters analysis system design and other aspects of the system are introduced. This system has the character-istics of simple,high-consistency and high-throughput. System test experiments and cell experiments were carried out to determine the basic performance of the system. Experimental results show that the cellular growth and cardio-myocyte beating can be detected by cellular multiphysiological parameter analysis system with rapid, long-term, high-throughput and non-invasive measurement of characteristics. It will provide a utility platform for the cellular physiological study.

关 键 词：生物医学 细胞传感器 阻抗传感器 细胞多生理参数分析系统 细胞生长和搏动 小鼠脑神经瘤细胞细胞 原代新生大鼠心肌细胞 

分 类 号：TP216[自动化与计算机技术—检测技术与自动化装置]