珍贵阔叶树种刺楸离体繁殖技术  

Propagation in vitro techniques of rare broad-leaved species of Kalpanax septemlobus

作　　者：张丽杰[1] 赵丽蒙 韩冬雪[1] 白雪娇[1] 周永斌[1] 

机构地区：[1]沈阳农业大学林学院,辽宁沈阳110866

出　　处：《经济林研究》2014年第4期127-134,共8页Non-wood Forest Research

基　　金：国家科技支持计划专题(2012BAD22B040206);林业公益性行业项目"辽宁省宜林地营林决策平台构建及示范"(201304216)

摘　　要：为建立刺楸组织培养体系,选取刺楸成熟胚、休眠芽及幼嫩茎段进行离体培养,探讨不同消毒时长、培养基种类及激素浓度对刺楸离体快繁的影响。结果表明:由于刺楸种子为胚乳性种子,因此选用刺楸的成熟胚进行离体培养,无论采用何种消毒方式均未获得成功;而休眠芽及幼嫩茎段分别使用0.1%的Hg Cl2消毒12、10 min时灭菌效果最佳;在对休眠芽和幼嫩茎段进行离体培养时筛选出1/2MS+6-BA2.0 mg/L+NAA0.1 mg/L为最适培养基及激素组合,腋芽萌发率可以达到66.7%和88.5%;生根培养时1/2MS+NAA0.1 mg/L+20 g/L蔗糖为最适培养基;移栽后1周,生长正常,长势良好。In order to establish a tissue culture system of Kalopanax septemlobus, taking mature embryos, dormant buds and tender stem segments in K. septemlobus as materials, the effects of different disinfection time, media types and hormone concentrations on propagation in vitro of broad-leaved K. septemlobus were researched. The results showed that because of the endosperm of seeds, propagation in vitro failed with any disinfection methods when mature embryos were taken materials. However, the sterilization effects of 0.1%HgCl2 for 12 rain and 10 min respectively were the best when dormant buds and tender stem segments were taken materials. The optimal medium and hormone combination was 1/2MS ＋ 6-BA2.0 mg/L ＋ NAA0.1 mg/L when dormant buds and tender stem segments were taken materials, and the germination rate of axillary buds was up to 66.7% and 88.5% respectively. The optimum medium for rooting culture was 1/2MS ＋ NAA0.1 mg/L ＋ 20 g/L sucrose. One week later, the transplanted seedlings grew well.

关 键 词：珍贵阔叶树种 刺楸 离体繁殖 

分 类 号：S685[农业科学—观赏园艺]