利用微卫星标记鉴别油茶品种  

A case study on cultivar identification in Camellia L. by using SSR markers

作　　者：陈赢男[1] 张新叶[1] 戴晓港[1] 

机构地区：[1]南京林业大学林木遗传与基因工程重点实验室,江苏南京210037

出　　处：《经济林研究》2014年第4期140-143,共4页Non-wood Forest Research

基　　金：林业公益性行业科研专项(200904002);国家自然基金项目(31400564);江苏省自然基金项目(BK20130968);江苏省南方现代林业协同创新项目;江苏高校优势学科建设工程(PAPD)

摘　　要：为发展可靠的油茶品种遗传真实性鉴定技术,以浙江红花山茶等油茶品种为试材,利用聚丙烯酰胺凝胶电泳对大量油茶SSR引物进行筛选,获得了电泳指纹清晰、基因型容易判定且多态性信息含量高的引物组合10对,提出了详细的用于油茶品种鉴别的试验操作流程。通过针对性的试验实例,验证了所开发引物应用于油茶品种遗传鉴别的有效性。In order to establish a set of reliable techniques for verifying the authenticity of Camellia L. cultivars, taking Camellia chekiangoleosa and some other cultivars as materials, a large amount of Camellia L. SSR primers were tested using polyacrylamide gel electrophoresis. Ten pairs of primers with clear polymorphic fingerprint, easy identifying genotype and high polymorphic information content, and the detailed test operation process for cultivar identification in Camellia L. was presented. Efficiency of the established system for identifying the authentic cultivar in Camellia L. was demonstrated through a pointed case study.

关 键 词：油茶 品种鉴别 微卫星标记 

分 类 号：S603[农业科学—园艺学] S794.4