毛菍茎段离体培养  

Stem segment in vitro culture in Melastoma sanguineum Sims

作　　者：伍成厚[1] 郑慈真 代色平[1] 孙砥柱 刘文[1] 黄春燕[1] 

机构地区：[1]广州市园林科学研究所,广东广州510405

出　　处：《经济林研究》2014年第4期147-151,共5页Non-wood Forest Research

基　　金：广州市科技计划项目(11A63030233)

摘　　要：为加速优良母株的无性繁殖，以嫩梢茎段为材料，进行了毛惹快繁技术的研究。结果表明：离体培养的毛葱茎段腋芽萌发后可以形成丛生芽，MS培养基添加6-BA013～2．0mg／L时芽增殖系数达到3．6～6．5倍；在培养基1／2MS＋NAA0．1～0．5mg／L中试管苗生根率均达到100％；在水帘温室内只需常规管理，试管苗移栽成活率超过95．0％。培养基添加300mg／L活性炭对毛慈芽的增殖和根的诱导均产生不利影响。In order to accelerate asexual propagation of superior mother plant, using tender stem segments as materials, rapid propagation in Melastoma sanguineum Sims was studied. The results showed that cluster buds could form after axillary buds in stem segments germinating, and multiplication coefficient reached 3.6-6.5 times when 0.3-2.0 mg/L 6-BA was added into MS medium. The rooting rate of test-tube plantlets reached 100% in the 1/2 MS added with 0.1-0.5 mg/L NAA. The survival rate of transplanted test-tube plantlets was over 95.0% through normal management in the greenhouse with water curtain. Adding activated carbon 300 mg/L into the media would reduce the rates of bud multiplication and root inducing in M. sanguineum Sims.

关 键 词：毛菍 茎段 不定芽 试管苗 快速繁殖 

分 类 号：S685[农业科学—观赏园艺]