桑椹补脑膏对东莨菪碱所致记忆获得障碍小鼠学习记忆能力影响  

Effects of Sangshen Bunao Creams on Learning and Memory Acquired Disorder Induced by Scopolamine in Mice

作　　者：刘瑾[1] 刘永伟 李岩[1] 于蓓蓓[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]威海市文登区小观镇卫生院,山东威海264400

出　　处：《辽宁中医药大学学报》2015年第1期22-24,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划项目(2011-164)

摘　　要：目的:研究桑椹补脑膏对东莨菪碱所致记忆获得障碍小鼠学习记忆的影响并初步探讨其作用机制。方法:KM小鼠60只,随机分为6组,即空白对照组,模型对照组,阳性对照药吡拉西坦1.4 g/kg组,桑椹补脑膏1.3、2.6、3.9 g/kg组,每组10只。灌胃给予等容积药物或生理盐水,每日1次,连续35 d。于末次给药后1 h,东莨菪碱造模,采用跳台法测试小鼠学习、记忆能力,采用比色法测定小鼠脑组织中超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量。结果:桑椹补脑膏能明显改善模型小鼠的学习能力和记忆能力,提高小鼠脑组织中SOD活性,降低MDA含量。结论:桑椹补脑膏能明显改善东莨菪碱所致小鼠学习记忆障碍,其作用机制可能和提高脑内SOD活性、降低MDA含量有关。Objective ： To study the effects of Sangshen Bunao Creams （ SSBNG ） on learning and memory ability in acquired disorder induced by Scopolamine and its preliminary mechanism. Methods ： Sixty Kunming mice were random!lY divided into control group, model group, SSBNG low, middle and high dose groups （ 1.3,2.6,3.9 g · kg-1 ）, ten mice in each group. The mice were administrated once per day and 35 days later, Scopolamine was injected intraperitoneally to induce learning and memory acquired disorder in mice. The step-down test was adopted to reflect the learning and memory of mice and the colorimetric method was used to determine the enzyme activity of superoxide dismutase （ SOD ） and the content of malondialdehyde （ MDA ） in the brain tissue. Results ： SSBNG could significantly promote the learning and memory capability of mice, increase the activities of SOD and reduce the contents of MDA in the brain tissue. Conclusion ： SSBNG could improve the ability of learning and memory of model mice and its mechanism should be related to increasing the activities of SOD and decreasing the contents of MDA in the brain tissue.

关 键 词：桑椹补脑膏 东莨菪碱 学习记忆 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]