哈蟆油对雌性衰老大鼠子宫组织p21和cyclinD1基因表达研究  

Study on Expression of Uterus Tissue p21 and cyclin D1 Gene in Aged Female Rats with Oviductus Ranae

作　　者：范青红[1] 姚晖[1] 郑红丽[1] 李晓莹[1] 黄志恩[1] 谢伟贤[1] 

机构地区：[1]佛山市第二人民医院,广东佛山528000

出　　处：《辽宁中医药大学学报》2015年第1期30-32,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广东省中医药管理局科研课题(20111059);佛山市科技发展专项资金项目(2011AA100011);佛山市医学类科技攻关项目(201108046)

摘　　要：目的:探讨哈蟆油(OR)对D-半乳糖所致雌性衰老大鼠子宫组织细胞增殖调控因子p21和cyclin D1基因表达的影响,进一步探讨OR延缓雌性大鼠生殖器官衰老机制。方法:SPF级SD雌性青年大鼠40只随机分为模型组(D-gal组)、维生素E(VE组)、哈蟆油高剂量组(OR-H组)、中剂量组(OR-M组)、低剂量组(OR-L组),每组8只,D-半乳糖颈背部皮下注射42 d,建立亚急性衰老模型。另取雌性青年大鼠8只,同样部位每日注射生理盐水,作为空白组。第15天开始灌胃给药,给药时间28 d。给药结束后,实时荧光定量PCR(q RT-PCR)法检测衰老大鼠子宫组织p21和cyclin D1基因的表达情况。结果:雌性衰老大鼠各组子宫组织q RT-PCR结果显示p21和cyclin D1基因表达相比均有显著的统计学意义(P<0.01)。D-gal组p21基因表达与Normal组比较升高,差异有显著性(P<0.01)。OR各剂量组p21基因表达与D-gal组比较下降,差异有显著性(均P<0.01),OR-L组作用较为明显。D-gal组cyclin D1基因表达与Normal组比较降低,差异有显著性(P<0.01);OR各剂量组cyclin D1基因表达与D-gal组比较均升高,差异有显著性(均P<0.01),表达随着OR剂量增加而升高。结论:OR可降低雌性衰老大鼠子宫组织细胞增殖负性调控因子p21基因表达,提高正性调控因子cyclin D1基因表达,促进衰老雌性大鼠子宫细胞增殖。OR延缓雌性生殖器官衰老作用可能通过调控子宫组织p21-cyclin D1信号通路发挥作用。Objective：To investigate the expressions of uterus tissue cellular proliferation regulating factor p21 and cyelinD1 in aged female rats with Oviductus Ranae （ OR ） by D-galactose. Methods ： 40 female SD rats （ SPF grade ） were randomly divided into 5 equal groups, namely the OR-H group, OR-M group, OR-L group, VE group and D-gal group. The rats received subcutaneous injection of D-Galactose for 42 d to establish aging models. Another 8 rats were injected daily with normal saline （ NS ） to serve as the normal control group. From the 15th of the experiment, the rats were given oral OR （ in OR group ）or Vitamin E （ in VE group ） for 28 days. After completion of drug administration, all the rats were sacrificed and p21 and cyclinD1 gene in uterus tissues were detected by qRT-PCR. Results： The result of qRT-PCR showed that gene expression of p21 in uterus of D-gal female group was marked increased than normal. All OR groups were significantly lower than D-gal group （ P〈0.01 ）. The expression of p21 gene in OR-L group was lowest in all OR groups. The result of qRT-PCR showed that gene expression of cyclinD1 in uterus of D-gal female group was markedly dencreased than normal. All OR groups were significantly higher than D-gal group （P〈0.01） and gene expression increased with OR＇s dose increasing. Conclusions ： After taking OR, OR can decrease over-expression of p21 gene and increase expression of eyclinD1 in uterus. OR can promote uterus cell proliferation of aged female rats. Anti-aging effects of OR is possibly through regulation of p21-eyclinD1 signaling pathways to promote expression of the proliferation regulatory protein and the signaling pathways play a role in delaying aging.

关 键 词：哈蟆油 衰老 子宫 D-半乳糖 

分 类 号：R965[医药卫生—药理学]