大孔树脂纯化沙苑子总黄酮工艺优选  

Optimization of Purification Technology for Total Flavonoids from Semen Astragali Complanati by Macroporous Adsorption Resin

作　　者：吴晓 周礼明 马威[2] 刘春宇[2] 

机构地区：[1]南京易亨制药有限公司,江苏南京210018 [2]苏州大学药学院,江苏苏州215123

出　　处：《辽宁中医药大学学报》2015年第1期54-57,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：江苏省高校自然科学基础研究项目(06KJB360105);苏州市科技计划项目(SYS201205)

摘　　要：目的:优选大孔吸附树脂纯化沙苑子总黄酮的工艺。方法:考察AB-8、D-101和NKA-9树脂对沙苑子总黄酮的吸附及解吸性能,筛选树脂型号;以总黄酮含量为指标,采用单因素试验和正交试验优选AB-8树脂纯化工艺条件。结果:选用AB-8型大孔吸附树脂,优选的工艺条件为:上样液质量浓度10 mg·m L-1,样品液p H 5.0,上样速度为1.0 m L·min-1,乙醇洗脱浓度40%,洗脱流速为1.5 m L·min-1;经AB-8处理后的沙苑子总黄酮纯度达50%。结论:AB-8型大孔吸附树脂能有效地纯化富集沙苑子总黄酮。Objective：To optimize the purification technology of total fIavonoids from the semen Astragali complanati by macroporous adsorption resin. Method： Static and dynamic adsorption and desorption performance of three kinds of macroporous adsorption resins （ AB-8, D-101, NKA-9 ）for total flavonoids from the semen Astragali complanati were investigated in order to select optimal resin. Purification conditions of AB-8 macroporous resin were optimized by single factor test and orthogonal experiment design. Result： AB-8 macroporous resin was chosen and its optimized technology conditions were as followings： the concentration of sample solution 1. 0 g· mL-1 （ pH=5 ）, the sample flow rate 1.0 mL ~ min-1, elution flow rate 1.5 mL · min-1 with 40 % ethanol. After purified with AB-8 macroporons resin, purity of total flavonoids was up to 50 %. Conclusion ： The total flavonids from the semen Astragali complanati can be effectively purificated by AB-8 macroporous resin.

关 键 词：大孔树脂 沙苑子总黄酮 纯化工艺 

分 类 号：R284[医药卫生—中药学]