SCCmec相关的psm-mec在血液来源人葡萄球菌中的基因定位  

Genetic location of SCCmec-associated psm-mec in Staphylococcus hominis from blood culture

作　　者：陈亮[1] 杨永长[2] 肖代雯[2] 喻华[2] 刘华[2] 黄文芳[2] 

机构地区：[1]泸州医学院临床医学院,四川泸州646000 [2]四川省人民医院检验科,四川成都610072

出　　处：《国际检验医学杂志》2015年第1期27-29,32,共4页International Journal of Laboratory Medicine

摘　　要：目的了解SCCmec相关的psm-mec基因在血液来源人葡萄球菌中的基因定位特征,为深入研究psm-mec基因在人葡萄球菌中的功能奠定基础。方法收集临床血培养分离的人葡萄球菌25株,通过PCR扩增mecA基因和psm-mec基因,多重PCR对耐甲氧西林人葡萄球菌SCCmec分型,PCR扩增mecR1/psm-mec基因与psm-mec/xylR基因间隔序列及fudoh基因,探究psm-mec基因在SCCmec上的定位特点。结果 PCR检测显示,21株为耐甲氧西林人葡萄球菌,psm-mec基因的检出率为47.6%,4株为甲氧西林敏感人葡萄球菌,未检出psm-mec基因。多重PCR结果显示,10株psm-mec基因阳性菌株中,2株属典型SCCmecⅢ型,5株属类SCCmecⅢ型,3株属SCCmec新型别。基因间隔序列和fudoh基因检测显示,10株psm-mec基因阳性人葡萄球菌中,mecR1/psm-mec基因和psm-mec/xylR基因及fudoh基因均阳性。结论 SCCmec相关的psm-mec基因广泛存在于血液来源的人葡萄球菌中,主要分布在典型SCCmecⅢ型、类SCCmecⅢ型和SCCmec新型别上,定位于mecR1基因与xylR基因之间。Objective To investigate the genetic location of SCCmec-associated psm-mec in Staphylococcus hominis isolated from blood culture,and to lay a foundation for further functional studies of psm-mec in Staphylococcus hominis. Methods 25 strains of Staphylococcus hominis isolated from positive blood culture were collected, mecA and psm-mec gene were amplified by PCR, and the SCCmec types were determined by the results of multiplex PCR assay. For analyzing the genetic location characteristic of psm-mec in SCCmec, three pair special PCR primers were used to measure mecR1/psm-mec, psm-mec/xylR and fudoh respectively. Results There were 21 strains of methicillin-resistant Staphylococcus hominis and 4 strains of methicillin-sensitive Staphylococcus hominis. The positive rate of psm-mec gene in methicillin-resistant Staphylococcus hominis was 47.6%, and no psm-mec gene was found in methicillin-sensitive Staphylococcus hominis. Among psm-mec positive strains,2 strains belonged to SCCmec Ⅲ, 5 strains belonged to SCCmec Ⅱ-like, and 3 strains belonged to new SCCmec types. All of the 10 psm-mec positive strains were mecR1/psm-mec, psmmec/xylR and fudoh gene positive. Conclusion SCCmec-associated psm-mec extensively exists in methicillin-resistant Staphylococcus hominis isolated from positive blood culture, which distributes mainly in typical SCCmec Ⅲ, SCCmec Ⅲ-like and new SCCmec types and locates between mecR1 and xylR gene.

关 键 词：SCCmec分型 psm-mec基因 人葡萄球菌 

分 类 号：R378.1[医药卫生—病原生物学]