慢性丙型肝炎患者血清HCV-RNA水平与抗-HCV抗体和肝纤指标水平相关性分析  

Study on correlation between serum HCV-RNA level and Anti-HCV with liver fibrosis indexes in patients with chronic hepatitis C

作　　者：赵小英[1] 杨邵华[1] 

机构地区：[1]甘肃省中医院检验科,甘肃兰州730050

出　　处：《国际检验医学杂志》2015年第1期47-48,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨丙型肝炎患者RNA水平与抗-HCV抗体和肝纤指标水平的关系。方法收集96例慢性丙型肝炎患者的血清,实时荧光定量PCR测定HCV-RNA和ELISA法检测抗-HCV抗体。化学发光法检测LN、PCⅢ、CⅣ及放免法检测HA。并对抗-HCV抗体、HCV-RNA水平与血清肝纤指标之间的关系进行分析。结果本研究的96例抗-HCV抗体阳性的慢性丙型肝炎患者中,HCV-RNA阳性52例,阳性率为54.2%。随着抗-HCV抗体的S/CO值升高,HCV-RNA检出率也在增高,分别为9.5%、37.1%和92.5%。高病毒载量组与低病毒载量组比较,血清HA、LN、PCⅢ及CⅣ水平的差异无统计学意义(P>0.05)。结论慢性丙肝患者HCV-RNA阳性检出率与抗-HCV抗体的S/CO值有关,S/CO值越高,HCV-RNA阳性率越高。而RNA水平与肝纤指标水平没有相关性。Objective To investigate the correlation between serum HCV-RNA level and anti-HCV with liver fibrosis indexes in the patients with chronic hepatitis C. Methods The serum samples were collected in 96 cases of chronic hepatitis C. The HCV-RNA level was determined by real time fluorescence quantification PCR and Anti-HCV was detected by ELISA. LN,PCⅢ and CⅣ levels were detected by the electrochemical luminescence analyzer and HA was examined by radioimmunoassay（RIA）. The correlation between the HCV-RNA and Anti-HCV levels with the liver fibrosis indexes was analyzed with the statistic software. Results Among these 96 cases of Anti-HCV positive chronic hepatitis C, 52 cases （54.20%） were HCV-RNA positive. With increasing of the S/CO value of Anti-HCV,the detection rate of HCV-RNA was increased, which were 9.5 %, 37.1% and 92.5 %. The differences in HA, LN, PCⅢ and CⅣ levels between the high viral load group and the tow viral load group had no statistical significance （P〉0.05）. Conclusion The positive detection rate of HCV-RNA is related with the S/CO value of Anti-HCV,the higher the S/CO value, the higher the positive detection rate of HCV-RNA. But the HCV-RNA level has no correlation with the liver fibrosis indexes.

关 键 词：肝炎抗体 丙型 肝纤指标 

分 类 号：R512.63[医药卫生—内科学]