Cx37和Cx40在自发性高血压大鼠不同动脉间的表达差异  

Difference expression of Cx37 and Cx40 of arteries in spontaneously hypertensive rats

作　　者：潘立君[1] 李新芝[2] 卓泓宇 李丽[1] 魏丽丽[1] 于秀石[1] 司军强[1] 马克涛[1] 

机构地区：[1]石河子大学医学院生理学教研室,新疆维吾尔自治区石河子832002 [2]石河子大学医学院病理生理学教研室,新疆维吾尔自治区石河子832002

出　　处：《重庆医学》2015年第1期4-6,共3页Chongqing medicine

基　　金：国家重点基础研究发展计划(973计划)(2012CB52660011);国家自然科学基金资助项目(31260247);兵团医药专项基金资助项目(2012BA021);石河子大学杰出青年科技人才培育计划项目(2012ZRKXJQ01)

摘　　要：目的观察自发性高血压和正常血压大鼠肠系膜动脉和主动脉上连接蛋白(Cx)表达的变化。方法应用RT-PCR和Werstern blot技术比较自发性高血压大鼠(SHR组)和正常血压大鼠(对照组)肠系膜动脉和主动脉上Cx37和Cx40的mRNA和蛋白表达水平。结果 SHR组肠系膜动脉Cx37mRNA的表达降低(P<0.05),主动脉降低更为显著(P<0.01);肠系膜动脉Cx40mRNA的表达显著降低(P<0.01),而主动脉则没有变化(P>0.05)。SHR组肠系膜动脉Cx37蛋白的表达降低(P<0.05),主动脉降低更为显著(P<0.01);肠系膜动脉Cx40蛋白的表达降低(P<0.05),而主动脉则没有变化(P>0.05)。结论高血压可能通过降低血管细胞上Cx37和(或)Cx40的表达造成细胞间缝隙连接通讯异常。Objective To investigate the change of connexin （Cx） in mesenteric artery （MA） and aorta of spontaneously hypertensive rats （SHR） and normotensive rats .Methods Quantitative RT-PCR and Werstern blot technique were used to compare the difference in the expression of Cx37 and Cx40 mRNA and protein in MA and aorta of SHR and normotensive rats .Results The level of Cx37 mRNA expression in MA from SHR was decreased compared with that of the normotensive rats （P〈0 .05） ,and more significantly in aorta （P〈0 .01） .Cx40 mRNA expression in MA from SHR was significantly decreased compared with that of the normotensive rats （P〈0 .01） ,but no change in aorta （P〉0 .05） .The expression of Cx37 protein in MA from SHR was decreased compared with that of the normotensive rats （P〈0 .05） ,and more significantly in aorta （P〈0 .01） .Cx40 protein expression in MA from SHR was significantly decreased compared with that of the normotensive rats （P〈0 .05） ,but no change in aorta （P〉0 .05） . Conclusion Hypertension may could decrease gap junctional communication in cells of MA and aorta from SHR by the downregulation of the expression of Cx37 and/or Cx40 .

关 键 词：大鼠 近交SHR 肠系膜动脉 主动脉 电突触 连接蛋白 

分 类 号：R544.1[医药卫生—心血管疾病] R331.3[医药卫生—内科学]