青蒿素对肝癌细胞株HepG2细胞增殖的影响  

The effect of artemisinin on the proliferation of human hepatoma cell line HepG-2

作　　者：黄俊玲[1] 李广志[1] 黄赞松[1,2] 尹毅霞[1] 周喜汉[1] 覃月秋[1] 

机构地区：[1]右江民族医学院消化疾病研究所/附属医院消化内科,广西百色533000 [2]广西医科大学研究生学院,南宁530021

出　　处：《重庆医学》2015年第1期21-23,共3页Chongqing medicine

基　　金：广西自然科学基金课题(桂科自0542119);广西医疗卫生重点科研课题(桂卫重200887);广西中医药管理局课题(桂卫中gzzc0955)

摘　　要：目的探讨青蒿素对细胞株HepG2细胞增殖的影响。方法采用MTT法检测青蒿素对HepG2细胞作用后的增殖抑制率,流式细胞术检测药物作用HepG2后细胞周期及细胞凋亡的情况。结果 80μmol/L的青蒿素对肝癌细胞株HepG2具有增殖抑制作用,其抑制率呈剂量、时间依赖效应,可使HepG2细胞周期阻滞于G0/S期,并诱导肝癌细胞发生凋亡。结论青蒿素能抑制肝癌HepG2细胞增殖。Objective To investigate the effect of artemisinin on the proliferation of human hepatoma cell line HepG-2 .Methods The inhibition effect of cell proliferation in human hepatocelluar carcinoma cell line HepG2 of artemisinin was detected by MTT test ,and the cell cycle and apoptosis were detected by Flow cytometry .Results Artemisinin at 80 umol/L could effectively inhibited the proliferation of HepG-2 cell in a dose-and time-dependent manner;the drugs could block cells at G0/S phase ,and induct the HepG-2 cell apoptosis .Conclusion Artemisinin could effectively inhibit the proliferation of HepG-2 cell .

关 键 词：青蒿素 肝肿瘤 流式细胞术 细胞周期 细胞凋亡 

分 类 号：R285[医药卫生—中药学]