猪源屎肠球菌tetM基因的检测  

Detection of tet M Gene in Enterococcus faecium Isolated from Pigs

作　　者：王玲飞[1] 马彩珲 苑丽[1] 吴华[1] 刘建华[1] 贺志沛[1] 胡功政[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《江西农业学报》2015年第1期93-96,共4页Acta Agriculturae Jiangxi

基　　金：河南省郑州市金水区科技公关项目(金科2012农业12)

摘　　要：用微量肉汤倍比稀释法测定了猪源屎肠球菌对四环素等抗生素的敏感性,用PCR和DNA测序方法检测了屎肠球菌中四环素类耐药基因(tet基因),并分析了试验菌对四环素类的耐药机制。结果表明:14株猪源屎肠球菌对四环素、多西环素、环丙沙星、红霉素全部耐药,4株对头孢噻呋耐药;在14株屎肠球菌中,分别有13株和9株含tet L和tet M基因,其中有9株同时携带tet L和tet M基因;猪源屎肠球菌主要存在主动外排和核糖体保护两种耐药机制,其对四环素类的耐药性主要与携带tet M和tet L有关。The susceptibilities of pig-derived Enterococcus faecium to tetracycline and other antibiotics were detected by using broth microdilution method, and the prevalence of tetracyclines-tolerant genes （ tet genes） in Enterococcus faecium was investigated by using PCR and DNA sequencing.The aim of this study is to analyze the tolerant mechanisms of tested Enterococcus faecium strains to tetracyclines.The results showed that all 14 strains of Enterococcus faecium isolated from pigs were tolerant to tetracycline, doxycycline, ciprofloxacin and erythromycin, and 4 strains were tolerant to ceftiofur.Among the above 14 strains, 13 strains con-tained tetL gene, 9 strains contained tetM gene, and 9 strains carried both tetL and tetM.The tolerance of pig-derived Enterococcus faecium to tetracyclines was mediated mainly by two mechanisms： drug initiative excretion and ribosomal protection, and it was closely related to carrying tetM and tetL genes.

关 键 词：猪 四环素 耐药性 屎肠球菌 TETM基因 

分 类 号：Q78[生物学—分子生物学]