ISSR-Based Molecular Characterization of an Elite Germplasm Collection of Sweet Potato(Ipomoea batatas L.) in China  

作　　者：ZHANG Kai WU Zheng-dan LI Yan-hua ZHANG Han WANG Liang-ping ZHOU Quan-lu TANG Dao-bin FU Yu-fan HE Feng-fa JIANG Yu-chun YANG Hang WANG Ji-chun 

机构地区：[1]College of Agronomy and Biotechnology, Southwest University [2]Sweet Potato Engineering and Technology Research Center [3]Engineering Research Center of South Upland Agriculture,Ministry of Education [4]The Chongqing Three Gorges Academy of Agricultural Sciences [5]Nanchong Institute of Agricultural Sciences [6]College of Life Science, Southwest University

出　　处：《Journal of Integrative Agriculture》2014年第11期2346-2361,共16页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31101192);the Animal and Plant Breeding Project of Chongqing,China (cstc2010AB1053);the Application Development Key Project of Chongqing,China (cstc2013yykfb80010);the Fundamental Research Funds for the Central Universities,China (XDJK2011C004);the "111" Project (B12006) of Ministry of Education,China

摘　　要：To determine the genetic diversity and population structure of sweet potato accessions cultivated in China, and to establish the genetic relationships among their germplasm types, a representative collection of 240 accessions was analyzed using inter-simple sequence repeat （ISSR） markers. The mean genetic similarity coefifcient, Nei’s gene diversity, and shared allele distance of tested sweet potato accessions were 0.7302, 0.3167 and 0.2698, respectively. The 240 accessions could be divided into six subgroups and ifve subpopulations based on neighbor-joining （NJ） clustering and STRUCTURE results, and obvious genetic relationships among the tested sweet potato accessions were identiifed. The marker-based NJ clustering and population structure showed no distinct assignment pattern corresponding to lfesh color or geographical ecotype of the tested sweet potato germplasm. Analysis of molecular variance （AMOVA） revealed small but signiifcant difference between white and orange-lfeshed sweet potato accessions. Small but signiifcant difference were also observed among sweet potato accessions from the Southern summer-autumn sweet potato region, the Yellow River Basin spring and summer sweet potato region and the Yangtze River Basin summer sweet potato region. This study demonstrates that genetic diversity in the tested sweet potato germplasm collection in China is lower than that in some reported sweet potato germplasm collections from other regions. Pedigree investigations suggest that more diverse Chinese sweet potato varieties should be formed by broadening the selection scope of breeding parents and incorporating the introduced varieties into future breeding programs.To determine the genetic diversity and population structure of sweet potato accessions cultivated in China, and to establish the genetic relationships among their germplasm types, a representative collection of 240 accessions was analyzed using inter-simple sequence repeat （ISSR） markers. The mean genetic similarity coefifcient, Nei’s gene diversity, and shared allele distance of tested sweet potato accessions were 0.7302, 0.3167 and 0.2698, respectively. The 240 accessions could be divided into six subgroups and ifve subpopulations based on neighbor-joining （NJ） clustering and STRUCTURE results, and obvious genetic relationships among the tested sweet potato accessions were identiifed. The marker-based NJ clustering and population structure showed no distinct assignment pattern corresponding to lfesh color or geographical ecotype of the tested sweet potato germplasm. Analysis of molecular variance （AMOVA） revealed small but signiifcant difference between white and orange-lfeshed sweet potato accessions. Small but signiifcant difference were also observed among sweet potato accessions from the Southern summer-autumn sweet potato region, the Yellow River Basin spring and summer sweet potato region and the Yangtze River Basin summer sweet potato region. This study demonstrates that genetic diversity in the tested sweet potato germplasm collection in China is lower than that in some reported sweet potato germplasm collections from other regions. Pedigree investigations suggest that more diverse Chinese sweet potato varieties should be formed by broadening the selection scope of breeding parents and incorporating the introduced varieties into future breeding programs.

关 键 词：sweet potato genetic diversity population structure agro-ecological zone lfesh color ISSR 

分 类 号：S531[农业科学—作物学]