7个家蚕新品系对BmNPV的抵抗力及中肠组织中Bmlipase-1的表达水平检测  

Resistance of Seven New Bombyx mori Strains to Bm NPV and Detection of Bmlipase-1 Expression Level in Midgut Tissue

作　　者：肖阳[1] 吴福泉[1] 李庆荣[1] 叶明强[1] 邢东旭[1] 李丽[1] 罗国庆[1] 杨琼[1] 

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所,广州510610

出　　处：《蚕业科学》2014年第6期1006-1010,共5页ACTA SERICOLOGICA SINICA

基　　金：广东省农业攻关项目(No.2012B020304003);广东省部产学研结合科技创新平台建设专项(No.2012B090600049);国家科技部对外合作项目(No.KY-2012-10-05)

摘　　要：为了获得对家蚕核型多角体病毒(Bm NPV)感染抵抗力较强的家蚕品系,以现行推广杂交组合粤蚕6号和两广二号的8个亲本品种为材料,给3龄起蚕添食浓度为4×107m L-1的Bm NPV多角体悬液,连续12代接种病毒筛选后获得7个对Bm NPV的抵抗能力有显著提高的新品系,其中新品系研7-N感染Bm NPV的发病率由原品种的96.30%降低至51.46%,表现出极显著差异(P<0.01)。采用荧光定量PCR方法,检测新品系及其杂交组合幼虫中肠组织中与Bm NPV抗性相关的脂肪酶1(Bmlipase-1)基因的表达水平,结果显示不同新品系间Bmlipase-1基因的表达水平差异较大,与其对Bm NPV的抵抗能力一致,其中春5-N、研7-N和湘晖-N等新品系及其杂交组合的Bmlipase-1表达水平均较原品种及其杂交组合极显著上升(P<0.01)。获得的家蚕新品系可作为抗Bm NPV育种的优良素材。In order to obtain silkworm（ Bombyx mori） strains resistant to B. mori nucleopolyhedrovirus（ Bm NPV）,we took8 parental silkworm varieties of currently popularized hybrid combinations Yuecan No. 6 and Liangguang No. 2 as materials,fed the 3rd instar newly exuviated larvae with mulberry leaves treated by Bm NPV polyhedron suspension at concentration of 4 × 10^7 m L^- 1for 12 generations successively. 7 new silkworm strains with significantly increased resistance to Bm NPV were obtained,of which Bm NPV infection rate of Yan 7-N was reduced from 96. 30%（ the original strain） to 51. 46%（ the newly bred strain）,which showed extremely significant difference（ P〈 0. 01）. The expression level of Bmlipase-1 in midgut of the newly bred strains and their hybrid combinations was detected by fluorescence quantitative PCR. The results showed that the expression level of Bmlipase-1 varied greatly among different varieties,being consistent with their resistance to Bm NPV. Expression of Bmlipase-1 in newly bred strains Chun 5-N,Yan 7-N,Xianghui-N and their hybrid combinations was extremely significant higher than that of the original strains and their hybrid combinations（ P〈 0. 01）. The newly bred silkworm strains can be used as excellent materials for anti-Bm NPV breeding.

关 键 词：家蚕核型多角体病毒 抗性家蚕品系 食下感染 脂肪酶1基因 荧光定量PCR 

分 类 号：S882.1[农业科学—特种经济动物饲养] Q786[农业科学—畜牧兽医]