硝普钠对干旱胁迫下葡萄抗旱生理的影响  

Effects of sodium nitroprusside on drought-resistance physiology in grape under drought stress

作　　者：张永福[1] 何田方 牛燕芬[1] 彭声静[1] 莫丽玲[1] 杨雪[1] 陈宗国[1] 

机构地区：[1]昆明学院农学院,昆明650214 [2]陆良县农业局,云南陆良655699

出　　处：《广东农业科学》2014年第24期40-44,共5页Guangdong Agricultural Sciences

基　　金：云南省教育厅科学研究基金(2012Z097);昆明学院引进人才科研项目(YJL11030)

摘　　要：以玫瑰蜜葡萄为试材,以硝普钠(SNP)为NO供体,通过无土栽培的方法在营养液中加入20%PEG-6000进行模拟干旱,研究SNP对干旱胁迫下葡萄苗抗旱性生理的影响,探讨外源NO缓解干旱胁迫对葡萄伤害的相关机制。结果表明,在PEG-6000模拟干旱胁迫的条件下,SNP处理可使葡萄叶片的MDA含量、超氧阴离子产生速率、过氧化氢含量和相对电导率明显下降,而超氧化物歧化酶(SOD)和过氧化物酶(POD)活性明显提高,游离脯氨酸和可溶性蛋白质等渗透调节物质含量也明显上升,叶片含水量的下降速率则明显减缓;SNP处理还显著提高了干旱胁迫后葡萄的根系活力,并降低了根系MDA含量。Meiguimi grape was used as material, using SNP as NO donor, by soilless cultivation methods, and adding20% PEG-6000 in nutrient solution simulated drought. The influences of SNP on drought-resistance physiology in grape seedlings under drought stress, and the mechanism of exogenous NO alleviating the damage of drought stress on grape were investigated. The results showed that under PEG-6000 simulation drought conditions, SNP treatment could make the MDA content, production rate of superoxide anion radical, hydrogen peroxide content and relative electrical conductivity decreased significantly, while the activities of SOD and POD increased in leaf; the osmotic adjustment substances contents of free proline and soluble protein significantly increased in leaf, and water content decline rate was slow significantly. In addition, SNP treatment also significantly made the root activity increased and the MDA content in root decreased after drought stress.

关 键 词：葡萄 干旱胁迫 硝普钠 抗旱生理 聚乙二醇 

分 类 号：S663.1[农业科学—果树学]