海南粗榧细胞悬浮培养体系的建立  

Establishment of cell suspension culture system for Cephalotaxus mannii

作　　者：陈林[1] 李永成[1] 

机构地区：[1]海南大学食品学院,海南海口570228

出　　处：《广东农业科学》2014年第24期54-57,62,共5页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(21166007)

摘　　要：以海南粗榧愈伤组织进行悬浮培养,考察了培养基、维生素、蔗糖浓度、p H值、接种量对海南粗榧细胞悬浮培养体系建立的影响,并利用HPLC技术检测海南粗榧悬浮细胞和培养基中生物碱的含量。结果表明：海南粗榧悬浮细胞系培养的最适条件：MS为基本液体培养基、添加蔗糖35 g/L＋VB1 10 mg/L、接种量6%、初始p H值5.5-7.0;发酵过程中每5 d调节p H值至6.0,有利于细胞的生长和产物的合成;细胞的生长呈“S”型曲线,细胞生长周期为30 d,产物含量在培养25 d时达到最大;细胞活力呈先急剧上升后缓慢下降的趋势。Using the callus of Cephalotaxus mannii for suspension cultivation, the factors influencing the establishment of C. mannii cell suspension culture system, including culture medium, Vitamin, sucrose concentration, p H, inoculum amount, were investigated. The alkaloid content in cell suspension culture system of C. mannii was determined by HPLC.The results showed that the optimal medium for the cell suspension culture was MS ＋35 g/L sucrose ＋10 mg/L VB1, the inoculum size was 6% and initial p H was 5.5-7.0. It was useful for cell growth and harringtonine formatio to adjust p H to6.0 every 5 days in the experiment. The cell growth basically accorded with the ＂S＂ curve in one culture cycle, the cycle was 30 days, the greatest products was obtained at the 25 th day. The cell viability sharply increased at first and gradually decreased in later.

关 键 词：海南粗榧 愈伤组织 悬浮培养 生物碱 三尖杉酯类碱 

分 类 号：Q813.1[生物学—生物工程]