应用ISSR技术分析杧果畸形病病原菌的遗传多样性  

Study on genetic diversity of Fusarium mangiferae from mango malformation disease by ISSR analysis

作　　者：柳凤[1,2] 欧雄常[1] 韦继光[2] 詹儒林[1] 常金梅[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室,广东湛江524091 [2]广西大学农学院,广西南宁530004

出　　处：《广东农业科学》2014年第24期79-82,87,共5页Guangdong Agricultural Sciences

基　　金：中央级非盈利性研究机构基本科研业务费研究专项(1630062014007);农业行业科研专项(201203092-3);四川省科技计划项目(2014NZ0026)

摘　　要：为探索我国杧果畸形病病原菌的遗传多样性,运用ISSR分子标记技术分析了采自四川攀枝花市和云南华坪县的38个杧果畸形病病原菌的遗传多样性。供试的38个菌株经14条特异性引物共扩增出72条带,其中52条带具有多态性,多态性比率为72.22%。38个菌株的相似系数值为0.5972-0.9862,变幅为0.3889,平均为0.7885。其中菌株MG16与MG17的相似系数最大、为0.9862;菌株MG07和MG33的遗传相似系数最小、为0.5972。利用UPGMA法进行聚类分析,38株菌株在相似系数为0.76水平上分为5类,菌株MG33、MG07和MG36分别形成单一分支,MG25和MG35聚合在一起;其余菌株分布在第Ⅴ类中。我国杧果畸形病病原菌Fusarium mangiferae存在丰富的种资资源,且F.mangiferae与采集地点、杧果品种之间无明显相关性。In order to explicit the genetic diversity of 38 strains Fusarium mangiferae from mango malformation disease from Panzhihua, Sichuan, and Huaping, Yunnan in China, the ISSR technique was used. 72 bands were amplified by 14 ISSR primers, with an average of 5.14 bands per primer. The size of the amplified products ranged from 300 bp to2 000 bp. The genetic similarities among isolates ranged from 0.5972 to 0.9862. Isolates MG16 and MG17 were the most similar（0.9862 genetic similarity）, whereas isolates MG07 and MG33 were the least similar（0.5972） and were grouped in separate clusters. At a genetic similarity of 0.76, the unweighted pair-group method with arithmetic mean analysis separated the isolates into five distinct clusters, comprising 33, two, one, one and one isolate, respectively. The ISSR data supported the conclusion that the isolates of F. mangiferae were not necessarily related by geographic origin, type of tissue or the mango cultivar from which they were isolated. This is the first report on the diversity of F. mangiferae species associated with mango malformation in China.

关 键 词：杧果畸形病 ISSR 遗传多样性 

分 类 号：S667.7[农业科学—果树学]