3种分子标记分析罗非鱼选育群体的多态性效率比较  

Polymorphism comparison of tilapia breeding groups by RAPD, AFLP and SSR

作　　者：颉晓勇[1] 钟金香[2] 李思发[3] 

机构地区：[1]中国水产科学研究院南海水产研究所/广东省渔业生态环境重点实验室,广东广州510300 [2]广东省水产技术推广总站,广东广州510220 [3]上海海洋大学/农业部淡水水产种质资源重点实验室,上海201306

出　　处：《广东农业科学》2014年第24期114-117,共4页Guangdong Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(2007TS04)

摘　　要：应用RAPD、AFLP、SSR等3种分子标记,对吉富品系尼罗罗非鱼选育群体的多态性进行了分析。在40条RAPD引物、19对SSR引物、64对AFLP引物中,具有多态性、重复性好的引物分别为17条、19对和8对,扩增出多态性条带数分别为35、92、181条。结果表明,RAPD、AFLP、SSR多态性条带比例分别为20.35%、79.64%和58.77%,平均每个(对)多态性引物可以扩增出多态性片段数目分别为2.06、4.84和22.63。说明SSR和AFLP是研究吉富罗非鱼选育群体更为有效的分子标记。RAPD, SSR and AFLP assay were applied in polymorphism analysis of Nile tilapia breeding groups. 17 RAPD primers, 19 SSR primer pairs and 8 AFLP primer combinations showed high polymorphism ratio and good repeatability on Nile tilapia among 40 RAPD primers, 19 SSR primer pairs and 64 AFLP primer combinations, respectively.The percentages of polymorphic bands generated by RAPD, SSR and AFLP were 20.35%, 79.64% and 58.77%,respectively. The average numbers of polymorphic fragments generated by RAPD, SSR and AFLP primers were 2.06, 4.84 and 22.63, respectively. Based on these results, SSR and AFLP were more effective molecular markers for polymorphism analysis than RAPD in Nile tilapia.

关 键 词：罗非鱼 分子标记 多态性 比较 

分 类 号：Q347[生物学—遗传学] S917.4[农业科学—水产科学]