家兔UCP1第3外显子基因多态性研究  

Study on the SNP of UCP1 Exon3 in rabbits

作　　者：李维[1,2] 谢海强 刘若余[1,2] 林家栋[1,2] 李思[1,2] 杜雪琴[3] 肖超能 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室 [2]贵州大学动物科学学院/贵州省动物遗传育种与繁殖重点实验室,贵州贵阳550025 [3]贵阳市小河第三实验学校,贵州贵阳550006

出　　处：《广东农业科学》2014年第24期138-141,共4页Guangdong Agricultural Sciences

基　　金：贵州省科技支撑计划项目(黔科合NY字[2012]3013号)

摘　　要：为了解兔肉质性状特征,给兔的品种选种选育提供理论依据,选择UCP1基因设计1对引物扩增其第3外显子及第2内含子部分序列,采用DNA池PCR反应,直接测序法快速检测比利时兔、新西兰兔和加利福尼亚兔3个兔品种UCP基因多态性。结果在新西兰兔中发现1个多态性位点(T387C),多态位点对UCP1基因的RNA结构产生影响,其最小自由能由-917.51 k J/mol变为-927.12 k J/mol,而其他2个品种没有检测到该突变。In order to deeply understand the meat quality and provide basic information and scientific proof for better heredity selection of rabbit, the complete sequences of exon3 and part of intron2 region in UCP1 of rabbit were obtained by designing one pair of specific primers. DNA pools PCR and the direct sequencing were used to test the SNP site in Belgian rabbit, New Zealand rabbit and California rabbit. The results showed that only one SNP（G387C） site was found in New Zealand rabbit, and the polymorphism led to the changes of RNA secondary structure, its minimum free energy changed from-917.51 k J/mol to-927.12 k J/mol. However, there was no SNP site in the other two breeds.

关 键 词：UCP1 比利时兔 新西兰兔 加利福尼亚兔 多态性 

分 类 号：S829.1[农业科学—畜牧学] Q346.5[农业科学—畜牧兽医]