海南龙血竭HPLC分析方法及三种黄酮成分含量的测定  

HPLC analysis method of Dragon's Blood from Dracaena cambodiana and Simultaneous Quantitative Determination of Three Flavonoid

作　　者：王佳媛[1,2] 戴好富[2] 王辉[2] 彭世清[2] 梅文莉[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《时珍国医国药》2014年第12期2828-2830,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31070321);国家科技支撑计划项目课题(No.2013BAI11B04)

摘　　要：目的建立海南龙血竭的HPLC分析方法。方法采用对照品鉴定了17个化合物,并选取其中三种黄酮类成分进行含量测定。结果 4,4＇-二羟基-2-甲氧基二氢查耳酮、4,4＇-二羟基-2,6-二甲氧基二氢查耳酮、（2S）-7,3＇-二羟基-4＇-甲氧基黄烷浓度分别在10~50μg·ml-1（r=0.999 9）、24~120μg·ml-1（r=0.9995）、48~240μg·ml-1（r=0.999 8）内与各自峰面积积分值呈良好的线性关系;平均回收率分别为97.73%（RSD=0.02%）、97.46%（RSD=0.03%）、99.44%（RSD=1.12%）（n=5）;成分含量分别为为2.2,1.0,2.2 mg/g。结论该方法精密度和重现性好,可用于海南龙血竭的质量控制。Objective The HPLC analysis method of Dragong blood from Dracaena cambodiana was extablished. Methods The total of 17 compounds were identified by standard substance, and three of the flavonoids were quantiative analyzed. Results The calibration curves were linear within the range of 10 μg·ml^-1 - 50μg · ml ^- 1 （ r = 0. 999 9 ） for 4,4＇- dihydroxy - 2 - methoxy - dihydroehalcone ,24μg·ml^-1- 120μg·ml^-1 （ r = 0. 999 5 ） for 4,4＇- dihydroxy - 2,6 - dimethoxydihydrochalcone, and 48 μg·ml^-1- 240μg·ml^-1 （ r = 0. 999 8 ） for （ 2s ） - 7,3 ＂- dihydroxy - 4 ＇- methoxyflavane, respectively. The average recoveries were 97.93% （ RSD = 0.02% ）, 97.46% （ RSD = 0.03 % ） and 99.44% （ RSD = 1.12% ） （ n = 5 ）, respectively. The ingredient con- tents were 2.2rag/g, 1.0mg/g and 2.2rag/g, respectively. Conclusion This method has good repeatability and high precision, and can be used for the quality control of Dragon＇s blood from Dracaena cambodiana.

关 键 词：血竭 海南龙血树 HPLC分析方法 黄酮 定量分析 

分 类 号：R284.1[医药卫生—中药学]