云南松松塔提取物对人肝癌细胞增殖的影响  

The effect of proliferation on hepatocellular carcinoma cells from pinecone extract of Pinus yunnanenis Fr.

作　　者：于冬梅[1] 李冬梅[1] 刘熙[1] 丁云[1] 李春艳[1] 

机构地区：[1]大理学院药学与化学学院,云南大理671000

出　　处：《时珍国医国药》2014年第12期2835-2837,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81260632);国家自然科学基金(No.81102350)

摘　　要：目的研究云南松松塔乙醇提取物(YN-DEF)体外对人肝癌细胞的作用。方法采用四甲基偶氮唑盐比色法(MTT法),观察云南松提取物对肝癌细胞(HEPG2、Huh-7)增殖的影响及对正常肝细胞(L-02)的作用。结果与阴性对照组比较,在浓度1000μg/ml,72h时,YN-DEF对HEPG2、Huh-7的抑制率分别为72.55%、60.66%,且抑制作用均呈浓度依赖性;正常肝细胞(L-02)与不同浓度为YN-DEF共同孵育72h后,高浓度下(1000μg/ml)的存活率为73.51%。结论云南松松塔乙醇提取物(YN-DEF)具有抑制肝癌细胞增殖的作用,高浓度下对正常肝细胞毒性小。Objective To study the proliferation effect on hepatocellular carcinoma cells with alcohol extract from pinecone of Pi- nus yunnanenis Ft. （ YN - DEF） in vitro. Methods To observe YN - DEF on the influence of hepatocellular carcinoma cells （ HEPG2, Huh - 7） and the effect of the normal human liver（ L - 02） cell by MTT method. Results To compare with the negative control group, under the concentration 1000μg/ml, the inhibition ratio of HEPG - 2, Huh - 7 proliferation with YN - DEF were resptctively 72.55% ,60.66% in 72h;After co -incubation with the normal human liver cell and the different concentrations YN - DEF , the livability of normal human liver cell was 73.51% under the concentration 1000 μg/ml. Conclusion It had a inhibition on hepatocellular carcinoma cells, and had low cytotoxicity with alcohol extract from pinecone of Pinus yunnanenis Ft.

关 键 词：云南松 松塔 提取物 抗肿瘤 

分 类 号：R962[医药卫生—药理学]