六味生物制剂有效组分片剂中马钱苷含量的高效液相色谱法测定  

HPLC Determination of Loganin in Biological Liuwei's Effective Components Tablet

作　　者：杨霞[1] 翁志平[1] 杨银凤[1] 丁建新[1] 陈凯[1] 赵越[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《时珍国医国药》2014年第12期2840-2841,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81374072)

摘　　要：目的建立HPLC法测定六味生物制剂有效组分片剂中马钱苷含量。方法采用Ultimate Platisil C18色谱柱（250mm×4.6 mm,5μm）,以乙腈-水（15∶85）为流动相,流速为1.0ml·min-1,检测波长236 nm,柱温为35℃。结果马钱苷在质量浓度为50~350μg·ml^-1内与峰面积呈良好的线性关系（r=1）,平均回收率（n=6）为98.8%,RSD为2.3%。结论该方法专属性强,快速简便,可作为六味生物制剂有效组分片剂中马钱苷含量测定的方法,为六味生物制剂有效组分片剂的质量控制提供参考。Objective To establish a method for the HPLC determination of Loganin in biological Liuwei＇s effective components tablet. Methods A Ultimate Platisil C 18 column （250mm × 4.6mm,5μm） was used with Acetonitrile - Water （ 15：85 ） as the mo- bile phase, the flow rate was 1. 0 ml · min^-1 with an injection volume of 10p,l, the detection wavelength was at 236nm and the column temperature was 35 ℃. Results The calibration curve was linear in the range of 50 -350μg·ml^-1 of loganin with correla- tion coefficient of 1. The average recovery was 98.8% with RSD being 2.3% （ n =6）. Conclusion The method is simple, rapid and reliable ,which can be regarded as a reasonable method for determination of loganin in biological Liuwei＇s effective components tablet. The results will supply reference for developing quality specification of biological Liuwei＇s effective components tablet.

关 键 词：六味生物制剂有效组分片剂 马钱苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]