女贞子指纹图谱研究及红景天苷、特女贞苷的含量测定  

Study on Fingerprint and Determination of salidroside,specnuezhenide of Ligustrum lucidum by HPLC

作　　者：崔蕴慧[1] 樊化[2] 利弈成 王治平[3] 

机构地区：[1]暨南大学附属第一医院,广东广州510632 [2]暨南大学生物医药研究院,广东广州510632 [3]广东药学院药科学院,广东广州510006

出　　处：《时珍国医国药》2014年第12期2854-2857,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家重大新药创制项目(No.2011ZX09401-307);广东省科技计划项目(No.2012A08204003)

摘　　要：目的建立女贞子药材的高效液相指纹图谱,并同时测定女贞子中红景天苷和特女贞苷的含量。方法采用高效液相法测定,色谱条件：Ultimate AQ C-18色谱柱（250 mm×4.6 mm,5μm）;流动相为乙腈-水,梯度洗脱;检测波长为275nm;体积流量为1.0 ml·min^-1;柱温为35℃。结果红景天苷、特女贞苷分别在进样量0.113 2~5.66μg（r=0.999 9）、0.146 9~7.345μg（r=0.999 8）范围内线性关系良好,平均回收率分别为98.3%、100.8%,按此方法建立了女贞子药材的HPLC指纹图谱,包含12个共有峰。结论该试验同时对女贞子的两种成分和指纹图谱进行分析,快速、简便、准确,为控制女贞子药材的质量提供了可靠的分析方法。Objective To develop a HPLC method of the chromatographic fingerprint of Ligustrum lucidum for quality control. Methods HPLC was used to determine the contents. The analytical column was Ultimate AQ C - 18 column （250 mm×4.6 mm,5μm）. The mobile phase was composed of acetonitrile （A） and water （B） with gradient elution. The detection wavelength was set at 275 nm ,the flow rate was 1. 0 ml·min^-1 and the column temperature was mainrained at 35 ℃. Results The fingerprint chro- matography included 12 mutual peaks. The linear ranges were in the range of 0. 113 2 -5.66 μg（ r = 0. 999 9） ,0. 146 9 -7. 345 μg（ r = 0. 999 8 ） for salidroside and specnuezhenide, respectively the average recoveries were 98.3% , 100.8%. Conclusion The method is rapidly, simple, accurate and can be used for the quality control of Ligustrum lucidum .

关 键 词：女贞子 指纹图谱 红景天苷 特女贞苷 

分 类 号：R284.2[医药卫生—中药学]