两种生化分析系统检测老年急性心肌梗死患者血清磷酸肌酸激酶同工酶的对比研究  

Study of comparable results of serum CK-MB in elderly patients with acute myocardial infarction by using two biochemical analysis system assessment

作　　者：魏小文[1] 胡卫红[1] 张薇[1] 刘运周[1] 李建业[1] 王超要[1] 王华[1] 曹鸿挺[1] 

机构地区：[1]中国人民解放军第421医院检验输血科,广州市510318

出　　处：《老年医学与保健》2014年第6期409-411,共3页Geriatrics & Health Care

摘　　要：目的 对比研究两种生化分析系统检测老年急性心肌梗死（AMI）患者血清磷酸肌酸激酶同工酶（CK-MB）的结果的一致性和可比性,以寻求准确与快速的检测方法.方法 选择80例老年AMI患者,随机分为校准前组和校准后组,每组40例.在同一实验室两种生化分析系统上对CK-MB进行方法对比试验,以美国贝克曼库尔特CX4 PRO为对比方法,以德国罗氏COBAS C50l为实验方法,检测患者血清中CK-MB活性,统计计算两方法间的相关系数和直线回归方程;然后以直线回归方程校准COBAS C501,再次检测患者血清中CK-MB活性,统计算两方法间的相关系数和直线回归方程.结果 校准前两分析系统CK-MB检测结果的预期偏差不能接受,校准后两分析统CK-MB检测结果的预期偏差可以接受.结论 同一实验室不同分析系统检测老年AMI患者血清CK-MB时应该进行方法对比实验和偏差评估,以确保检测结果的一致性.Objective To study the comparable results of serum CK-MB in elderly patients with acute myocardial infarction （AMI） by using two biochemical analysis system assessment in order to seek accurate and rapid detection method. Methods 80 elderly patients with AMI were randomly divided into two groups： before calibration group and after calibration group, 40 cases each group. CX4 PRO （comparison method） and COBAS C501 （laboratory method） were used to test the levels of serum CK-MB in patients to calculate the correlation coefficient and linear regression equation, respectively. The regression equation were used to calibrate the CK-MB outcome of COBAS C501. Then two different biochemical analysis systems were used to test the levels of serum CK-MB in patients to calculate the correlation coefficient and linear regression equation again. Results The outcome biases of CK-MB were not acceptable by clinic before calibrating, but the outcome biases of CK-MB were acceptable by clinic after calibrating. Conclusion In order to insure the accuracy and comparability of the test results, method comparison and bias estimation should be performed if two or more biochemical analysis systems will be used in the same clinical laboratory.

关 键 词：老年 急性心肌梗死 磷酸肌酸激酶同工酶 偏差 评估 全自动生化分析系统 

分 类 号：R542.22[医药卫生—心血管疾病]