Research on Callus Induction from Coleoptile Explants of Amorphophallus  

魔芋芽鞘外植体诱导愈伤组织研究(英文)

作　　者：胡选萍[1,2] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723000 [2]陕西省资源生物重点实验室,陕西汉中723000

出　　处：《Agricultural Science & Technology》2014年第12期2085-2087,2107,共4页农业科学与技术（英文版）

基　　金：Supported by Shaanxi University of Technology Scientific Research Program(SLGKY14-12);Natural Science Program of Education Department of Shaanxi Provincial Government(2013JK0741)~~

摘　　要：[Objective] The aim was to explore the ways of callus induction and the effect that different culture condition exerted on the callus induction from coleoptile explants of Amorphophallus. [Method] The test used konjac coleoptile as materials and designed four hormones, including 6-BA, NAA, KT and 2, 4-D, grouping 8 treat- ments to conduct statistics on callus induction in different test conditions. [Result] The result showed that multi-location generation and polymorphism was the impor- tant feature of callus induction from coleoptile explants of Amorphophallus; culture condition had significant influence on callus induction from coleoptile explants （P〈 0.01）. [Conclusion] It reached the best effect of callus induction when the culture condition was MS ＋ 1.0 mg/L 6-BA ＋ 2.0 mg/L NAA or MS ＋ 1.0 mg/LKT ＋ 2.0 mg/L 2, 4-D.[目的]探索魔芋芽鞘外植体愈伤组织的发生方式及不同处理条件对其愈伤诱导的效应程度,优化魔芋离体培养条件。[方法]以陕南地区魔芋芽鞘为试材,选用6-BA、NAA、KT、2,4-D 4种激素组合形成8种激素配比处理,统计不同处理条件下魔芋芽鞘愈伤组织的诱导情况。[结果]魔芋芽鞘外植体愈伤组织的发生表现出明显的多位点发生与多种不同形态;不同处理条件下魔芋芽鞘外植体脱分化启动与愈伤组织诱导效果存在显著差异(P<0.01)。[结论]当培养条件为MS+1.0mg/L 6-BA+2.0 mg/L NAA或MS+1.0 mg/L KT+2.0 mg/L 2,4-D时,魔芋芽鞘外植体脱分化诱导愈伤组织效果相对最佳。

关 键 词：AMORPHOPHALLUS COLEOPTILE CALLUS 

分 类 号：S632.3[农业科学—蔬菜学]