Enhanced Enzymatic Hydrolysis of Miscanthus sinensis Following Synergic Pretreatment with ^(60)Co γ-ray Irradiation and Alkaline Hydrogen Peroxide  

射线辐照和碱性双氧水协同预处理促进芒草酶解糖化的研究(英文)

作　　者：苏小军[1,2,3] 谭兴和[2] 彭姿[2] 胡秋龙[1] 熊兴耀[1,4] 

机构地区：[1]湖南农业大学湖南省作物种质创新与资源利用重点实验室,湖长沙410128 [2]湖南农业大学食品科技学院,湖南长沙410128 [3]湖南农业大学湖南省植物功能成分利用协同创新中心,湖南长沙410128 [4]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《Agricultural Science & Technology》2014年第12期2187-2191,共5页农业科学与技术（英文版）

基　　金：Supported by National High Technology Research and Development Program of China(863 Program)(2012AA101804)~~

摘　　要：[Objective] This study aimed to investigate the effects of different pretreat- ments on enzymatic saccharification of Miscanthus sinensis and improve reducing sugar yield in the enzymolysis process. [Method] M. sinensis was pretreated with 60Co y-ray irradiation and alkaline hydrogen peroxide, to analyze their effects on re- ducing sugar yield of enzymatic hydrolysis. [Result] After pretreatment with 400 kGy 60Co y-ray irradiation, reducing sugar yield in the enzymolysis process of M sinensis was 76.24 mg/g; after synergic pretreatment with 400 kGy 60Co y-ray irradiation and alkaline hydrogen peroxide, reducing sugar yield in the enzymolysis process of M. sinensis was 505.08 mg/g, which was improved by 5.6 times compared to that in pretreatment with 400 kGy 60Co y-ray irradiation. Based on process optimization, the optimal hydrolysis conditions were obtained： pretreatment temperature 30 ℃, NaOH concentration 1.2%, hydrogen peroxide concentration 2%, pretreatment time 6 h. [Conclusion] Synergic pretreatment with 60Co y-ray irradiation and alkaline hydrogen peroxide could significantly improve reducing sugar yield in the enzymolysis process of M. sinensis, which provided a new theoretical basis for preparing fuel ethanol with M. sinensis.[目的]为提高芒草的酶解产糖得率,研究不同预处理对其影响。[方法]采用射线辐照与碱性双氧水对芒草进行协同预处理,考察对其酶解产糖的影响。[结果]经400 k Gy剂量射线辐照预处理后,芒草酶解产还原糖的得率为76.24 mg/g;采用400 k Gy剂量射线辐照与碱性双氧水协同预处理,芒草酶解产还原糖的得率为505.08 mg/g,较只进行相同剂量射线辐照处理提高了5.6倍。通过工艺优化,得出较优水解条件为:预处理温度30℃、Na OH浓度1.2%、双氧水浓度2%、处理时间6 h。[结论]射线辐照与碱性双氧水协同预处理能够大大提高芒草酶解产糖效率,为利用其制备燃料乙醇提供了新的理论依据。

关 键 词：Miscanthus sinensis 60Co y-ray irradiation Alkaline hydrogen peroxide Enzymatic sacchadfication 

分 类 号：S578[农业科学—作物学]