“二神丸”中药物炮制前后对脾肾阳虚泄泻大鼠血清中一氧化氮和胃动素、胃泌素的影响  

The influence of “Ershen Wan” on NO,MTL and GAS in serum of the Spleen-Kidney Yang deficiency rats before and after processing

作　　者：潘新[1] 胡昌江[1] 耿媛媛[1] 赵玲[1] 熊瑞[1] 陈志敏[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2014年第5期26-28,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81274085)

摘　　要：目的：比较＂二神丸＂中两味药物炮制前后对脾肾阳虚泄泻模型大鼠血清中一氧化氮（NO）、胃动素（MTL）、胃泌素（GAS）含量的影响,拟阐明＂二神丸＂中药物炮制增强疗效作用机理。方法：将50只SD雄性大鼠随机分为正常、模型、阳性药物、生品＂二神丸＂、炮制品＂二神丸＂5组,通过复合因素造脾肾阳虚泄泻模型,并给药后取血清,用酶联免疫吸附法（ELISA）测定大鼠血清中一氧化氮、胃动素、胃泌素的水平。结果：模型组血清中一氧化氮、胃动素、胃泌素水平与正常组相比均有显著增高（P〈0.05）;三个给药组均能调节三者趋于正常水平,且炮制品＂二神丸＂组对一氧化氮、胃动素和胃泌素的调节作用比生品＂二神丸＂组更明显（P〈0.05～0.01）。结论：＂二神丸＂中的药物炮制后对大鼠血清中一氧化氮、胃动素和胃泌素的调节作用比生品＂二神丸＂组更为明显,提示复方＂二神丸＂中药物炮制后增强止泻作用可能是通过纠正异常的一氧化氮水平和消化道激素而达到的。Objective： To compare the influence of two medicines before and after processing in ＂Ershen Wan＂ on the content of NO, motilin（MTL）, gastrin（GAS） in serum of Spleen-Kidney Yang deficiency rats, and provide experimental basis for mechanism of enhancing curative effect after processing. Method： 50 SD male rats were randomly divided into 5 groups： normal, model, positive drug, ＂Ershen Wan＂, and ＂Ershen Wan＂ processed. Spleen-kidney yang deficiency diarrhea rats model were established with composite factors. The plasma from arteria cruralis was collected after treatment, and the contents of NO, MTL, and Gas were detected using ELISA method. Result： NO、MTL、Gas in model group were significantly increased compared with normal group（P〈0.05）. The positive, ＂ Ershen Wan＂ and ＂ Ershen Wan＂ processed groups can all significantly regulate the contents of NO, MTL, and Gas to normal level. Compared with ＂ Ershen Wan ＂ group, the impact on NO, MTL, and Gas was more significant in ＂ Ershen Wan ＂ processed group（P〈0.05～0.01）. Conclusion： The component medicine in ＂Ershen Wan＂ after processing can strength antidiarrheal effects which suggest that the mechanism may be related to the mediation by retrieving abnormal levels of NO and gastrointestinal hormone.

关 键 词：二神丸 炮制 一氧化氮 胃动素 胃泌素 

分 类 号：R285.5[医药卫生—中药学]