耐药型大肠杆菌引发的仔猪大肠杆菌病的PCR快速鉴定  

Rapid PCR Identification of Piglet Colibacillosis Caused by Drug Resistant Type Escherichia coli

作　　者：王选[1] 赵瑞利[1] 马吉飞[1] 胡烨[1] 路垚[1] 朱蕴暖 

机构地区：[1]天津农学院动物科学与动物医学学院,天津300384

出　　处：《天津农学院学报》2014年第4期26-28,共3页Journal of Tianjin Agricultural University

基　　金：天津市应用基础与前沿技术研究计划一般项目"新牛蛙抗菌肽RGD-嵌合体的分子设计及其靶向抗肿瘤研究"(14JCYBJC-30000);天津农学院科技发展基金计划项目"用GAP启动子在毕赤酵母中组成型表达牛蛙抗菌肽"(201202)

摘　　要：利用菌落PCR方法对临床致病菌进行快速鉴定,以天津地区某猪场病死猪胃、肠内容物作为病料进行细菌分离培养,并对所分离的细菌进行药敏试验鉴定。设计16S r RNA通用引物并利用菌落PCR方法扩增所分离细菌的16S r RNA序列,对该序列进行测定和BLAST分析。结果发现,该细菌为一株多重耐药性大肠杆菌。试验建立了仔猪大肠杆菌病的临床快速检测方法。In order to investigate the application value of polymerase chain reaction（PCR） technique in detection of pathogenic bacteria in piglets,pigs＇ stomach,intestinal contents as disease materials from a pig farm in Tianjin area were expected to isolate and cultivate the pathogenic bacteria and test the drug sensitivity.Universal primes were designed to detect 16 S r RNA sequences from isolated bacteria by using PCR method and the results were analyzed by the biological software BLAST.The results show that the pathogenic bacteria was a multiple drug resistant type Escherichia coli.A rapid clinical method of detecting colibacillosis of piglet was found in this study.

关 键 词：仔猪 耐药性 大肠杆菌 PCR BLAST分析 

分 类 号：S858.28[农业科学—临床兽医学]