人参多糖注射液对胃癌细胞SGC-7901凋亡的诱导作用及机制  

Ginseng polysaccharide injection induces apoptosis of gastric carcinoma SGC-7901 cells

作　　者：黄靓[1] 李国庆[1] 毛振江[1] 谷苗[2] 

机构地区：[1]南华大学附属第二医院消化内科,湖南省衡阳市421001 [2]南华大学附属第二医院急诊科,湖南省衡阳市421001

出　　处：《世界华人消化杂志》2014年第33期5114-5117,共4页World Chinese Journal of Digestology

摘　　要：目的:人参多糖注射液对胃癌细胞SGC-7901凋亡的诱导作用及机制.方法:采用不同浓度(0、12.5、25.0、50.0、100.0μL/m L)、不同时间(24、48、72 h)人参多糖注射液处理SGC-7901细胞,分别采用流式细胞仪及Western blot检测细胞凋亡及Bax、Bcl-2蛋白表达.结果:人参多糖注射液对SGC-7901细胞体外凋亡具有显著的促进作用,并呈现剂量及时间依赖性.人参多糖注射液处理后,SGC-7901细胞Bax蛋白表达及Bax/Bcl-2蛋白表达比值显著升高,Bcl-2蛋白表达显著降低,并呈现剂量及时间依赖性.结论:人参多糖注射液可诱导胃癌SGC-7901细胞凋亡,调节凋亡相关基因Bax、Bcl-2表达是其可能作用机制.AIM： To investigate the apoptosis-inducing effect of ginseng polysaccharide injection in gastric carcinoma SGC-7901 cells and the possible mechanism involved. METHODS： SGC-7901 cells were treated with different concentrations（0, 12.5, 25.0, 50.0, 100.0μL/m L） of ginseng polysaccharide injection for different durations（24, 48 or 72 h）. Cell apoptosis was assessed by flow cytometry, and protein expression of Bax and Bcl-2 was determined by Western blot.RESULTS： Ginseng polysaccharide injection significantly induced the apoptosis of SGC-7901 cells in vitro in a dose- and timedependent manner. Treatment with ginseng polysaccharide injection significantly in-creased Bax protein expression and Bax/Bcl-2 ratio, and reduced Bcl-2 protein expression in a dose- and time-dependent manner. CONCLUSION： Ginseng polysaccharide injection is able to induce apoptosis of gastric carcinoma SGC-7901 cells possibly by regulating Bax and Bcl-2 protein expression.

关 键 词：人参多糖注射液 胃癌 凋亡 BAX BCL-2 

分 类 号：R285[医药卫生—中药学]