加味藿香正气软胶囊对TNBS致小鼠结肠炎模型的抗炎作用  

Anti-inflammatory effects of Jiawei Huoxiang Zhengqi soft capsules in a mouse model of TNBS-induced colitis

作　　者：吕耀中[1] 王正宽[1] 孙兰[1] 郑伟然[1] 周军[1] 王振中[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,江苏省连云港市222001

出　　处：《世界华人消化杂志》2014年第33期5118-5123,共6页World Chinese Journal of Digestology

基　　金：国家国际科技合作专项基金资助项目;No.2013DFA31090~~

摘　　要：目的:研究加味藿香正气软胶囊(Jiawei Huoxiang Zhengqi soft capsule,JWHXZQ)对三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid TNBS)诱导小鼠结肠炎模型的抗炎作用及其机制.方法:将50只B a l b/c♀小鼠随机分为对照组、模型组、J W H X Z Q低、中、高剂量(0.234、0.468、0.936 g/kg)组.采用TNBS/乙醇灌肠制作小鼠结肠炎模型,同时开始每天ig给药,连续7 d.每天进行疾病活动指数(disease activity index,DAI)评分,7 d后处死小鼠,对各组小鼠结肠进行组织病理学评分检测血清中NO水平、结肠组织中髓过氧化物酶(myeloperoxidase,MPO)含量及白介素(interleukin,IL)-1β、IL-6、IL-12、肿瘤坏死因子(tumor necrosis factor,TNF)-α、干扰素(interferon,IFN)-γ等促炎性细胞因子水平.结果:与模型组比较,JWHXZQ中、高剂量组治疗可显著降低TNBS结肠炎小鼠的DAI评分和病理学评分,降低小鼠血清中NO水平,同时还显著降低结肠中MPO含量、IL-1β、IL-6、IL-12和TNF-α等促细胞因子水平;JWHXZQ低剂量组也能显著降低小鼠血清中NO含量、结肠中IL-1β和IL-6水平.结论:JWHXZQ对TNBS诱导的小鼠结肠炎具有一定治疗作用,其机制可能与抑制促炎性细胞因子产生及相关免疫应答通路而发挥作用.AIM： To assess the anti-inflammatory effects of Jiawei Huoxiang Zhengqi soft capsules（JWHXZQ） in mice with 2,4,6-trinitrobenzene sulfonic acid（TNBS） induced colitis and to explore the possible mechanisms involved.METHODS： Fifty female Balb/c mice were randomly divided into five groups： a control group, a model group, and low-, medium-, and high-dose JWHXZQ groups（0.234, 0.468, and 0.936 g/kg）.TNBS/ethanol was administered intracolonically to induce colitis in mice, and the corresponding drug was given by intragastric administration for 7 d at the same time. Disease activity index（DAI） was scored daily. Mice was killed on the 7th day, and the histopathological score for each mouse colon was evaluated. Serum NO level and the content of myeloperoxidase（MPO） in colonic tissue were measured. The levels of interleukin（IL）-1β, IL-6, IL-12, tumor necrosis factor（TNF）-α and interferon（IFN）-γ in colonic tissue were also measured.RESULTS： Compared with the model group, DAI scores, pathological scores, serum levels of NO, and the levels of MPO and cytokines including IL-1β, IL-6, IL-12 and TNF-α in the colon were significantly decreased in the medium- and highdose JWHXZQ groups. In the low-dose JWHXZQ group, serum level of NO as well as IL-1β and IL-6 levels in the colon were also significantly reduced compared with the model group.CONCLUSION： JWHXZQ has appreciated therapeutic effects against TNBS-induced colitis in mice, possibly via mechanisms related to the inhibition of inflammatory factors and associated immune response pathways.

关 键 词：加味藿香正气软胶囊 三硝基苯磺酸 结肠炎 炎症因子 

分 类 号：R285.5[医药卫生—中药学]