不同细菌及酵母菌对阴道毛滴虫体外生长的影响  

Influence of various bacteria and Yeast AH109 on growth of Trichomonas vaginalis in vitro

作　　者：丁鹤[1] 于艳辉[2] 宫鹏涛[1] 李建华[1] 杨举[1] 李赫[1] 张国才[1] 张西臣[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]吉林大学第二医院检验科,吉林长春130041

出　　处：《中国生物制品学杂志》2014年第11期1408-1411,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金项目(30970322)

摘　　要：目的研究大肠埃希菌J20、保加利亚乳杆菌、金黄葡萄球菌及AH109酵母菌体外对阴道毛滴虫生长的影响。方法分别将不同浓度(104、105、106和107个/ml)的大肠埃希菌J20、保加利亚乳杆菌、金黄葡萄球菌及AH109酵母菌与阴道毛滴虫进行体外共培养,并对不同培养时间(2、4、6和8 h)的虫体进行计数。结果与空白对照组相比,大肠埃希菌J20及金黄葡萄球菌在低浓度(104个/ml)时即明显影响阴道毛滴虫体外生长(P均<0.01),在6 h时即可见白色死亡虫体沉淀:保加利亚乳杆菌仅在高浓度(106和107个/ml)时对阴道毛滴虫的生长有明显影响(P均<0.01):AH109酵母菌在不同浓度及不同培养时间对阴道毛滴虫的生长均无明显影响(P>0.05)。结论大肠埃希菌J20、金黄葡萄球菌及高浓度(106和107个/ml)保加利亚乳杆菌对阴道毛滴虫生长影响显著,而AH109酵母菌无明显影响,为进一步研究阴道毛滴虫感染后,在体内与阴道菌群的相互作用关系奠定了理论基础。Objective To investigate the influence of Escherichia coli J20,Lacbobacillus bulgaricus,Staphylococcus aureus and Yeast AH109 on the growth of Trichomonas vaginalis in vitro. Methods Escherichia coli J20,Lacbobacillus bulgaricus,Staphylococcus aureus and Yeast AH109 at various concentrations（104,105,106 and 107 bacteria / ml） were co-cultured with Trichomonas vaginalis for various hours and counted for trophozoites. Results As compared with that in blank control group,the growth of Trichomonas vaginalis was influenced significantly by Escherichia coli J20 and Staphylococcus aureus at low concentrations（each 104 bacteria / ml）（each P 〈 0. 01）. White precipitate of dead trophozoites was observed 6 h after co-culture. Lacbobacillus bulgaricus at high concentrations（106 and 107 bacteria / ml）showed significant effect on the growth of Trichomonas vaginalis（each P 〈 0. 01）. However,Yeast AH109 at various concentrations and cultured for various hours showed no significant effect on the growth of Trichomonas vaginalis（ P 〉 0. 05）.Conclusion Escherichia coli J20,Staphylococcus aureus,as well as Lacbobacillus bulgaricus at high concentrations（106 and 107 bacteria / ml）showed significant effect on the growth of Trichomonas vaginalis,while Yeast AH109 showed no significant effect. It provided a theoretical basis for the study on interaction between Trichomonas vaginalis infection and vaginal flora in vivo.

关 键 词：阴道毛滴虫 大肠埃希菌J20 保加利亚乳杆菌 金黄葡萄球菌 AH109酵母菌 

分 类 号：R382.21[医药卫生—医学寄生虫学]