HPLC Pigment Profiles of 31 Harmful Algal Bloom Species Isolated from the Coastal Sea Areas of China  

作　　者：LIU Shuxia YAO Peng YU Zhigang LI Dong DENG Chunmei ZHEN Yu 

机构地区：[1]Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education [2]Ocean College,Zhejiang University [3]Qingdao Collaborative Innovation Center of Marine Science and Technology [4]Institute of Marine Organic Geochemistry,Ocean University of China [5]College of Chemistry and Chemical Engineering,Ocean University of China [6]Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology,State Oceanic Administration [7]Key Laboratory of Marine Environment and Ecology,Ministry of Education

出　　处：《Journal of Ocean University of China》2014年第6期941-950,共10页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 41176063 and 41221004);the National Marine Public Welfare Research Project of China (No. 201205031)

摘　　要：Chemotaxonomy based on diagnostic pigments is now a routine tool for macroscopic determination of the composition and abundance of phytoplankton in various aquatic environments. Since the taxonomic capability of this method depends on the relationships between diagnostic pigments and chlorophyll a of classified groups, it is critical to calibrate it by using pigment relationships obtained from representative and/or dominant species local to targeted investigation area. In this study, pigment profiles of 31 harmful algal bloom(HAB) species isolated from the coastal sea areas of China were analyzed with high performance liquid chromatography(HPLC). Pigment compositions, cellular pigment densities and ratios of pigments to chlorophyll a were determined and calculated. Among all these species, 25 kinds of pigments were detected, of which fucoxanthin, peridinin, 19'-butanoyloxyfucoxanthin, 19'-hexanoyloxyfucoxanthin, violaxanthin, and antheraxanthin were diagnostic pigments. Cellular pigment density was basically independent of species and environmental conditions, and therefore was recommended as a bridge to compare the results of HPLC-CHEMTAX technique with the traditional microscopy method. Pigment ratios of algal species isolated from the coast of China, especially the diagnostic pigment ratios, were higher than those from other locations. According to these results, pigment ratio ranges of four classes of phytoplankton common off the coast of China were summarized for using in the current chemotaxonomic method. Moreover, the differences of pigments ratios among different species under the same culturing conditions were consistent with their biological differences. Such differences have the potential to be used to classify the phytoplankton below class, which is meaningful for monitoring HABs by HPLC-CHEMTAX.Chemotaxonomy based on diagnostic pigments is now a routine tool for macroscopic determination of the composition and abundance of phytoplankton in various aquatic environments. Since the taxonomic capability of this method depends on the rela- tionships between diagnostic pigments and chlorophyll a of classified groups, it is critical to calibrate it by using pigment relation- ships obtained from representative and/or dominant species local to targeted investigation area. In this study, pigment profiles of 31 harmful algal bloom （HAB） species isolated from the coastal sea areas of China were analyzed with high performance liquid chro- matography （HPLC）. Pigment compositions, cellular pigment densities and ratios of pigments to chlorophyll a were determined and calculated. Among all these species, 25 kinds of pigments were detected, of which fucoxanthin, peridinin, 19＇-butanoyloxyfucoxan- thin, 19＇-hexanoyloxyfucoxanthin, violaxanthin, and antheraxanthin were diagnostic pigments. Cellular pigment density was basi- cally independent of species and environmental conditions, and therefore was recommended as a bridge to compare the results of HPLC-CHEMTAX technique with the traditional microscopy method. Pigment ratios of algal species isolated from the coast of China, especially the diagnostic pigment ratios, were higher than those from other locations. According to these results, pigment ratio ranges of four classes of phytoplankton common off the coast of China were summarized for using in the current chemotaxonomic method. Moreover, the differences of pigments ratios among different species under the same culturing conditions were consistent with their biological differences. Such differences have the potential to be used to classify the phytoplankton below class, which is meaningful for monitoring HABs by HPLC-CHEMTAX.

关 键 词：harmful algal bloom species  high performance liquid chromatography  chemotaxonomy  cellular pigment density  pigment ratios 

分 类 号：X55[环境科学与工程—环境工程]