C型凝集素受体和Toll样受体在原因不明复发性流产患者蜕膜中的表达  

Expression of C-type lectin receptors and Toll-like receptors in decidua of unexplained recurrent spontaneous abortion

作　　者：邱添[1] 滕银成[1] 王玉东[2] 徐亮[2] 

机构地区：[1]上海交通大学附属第六人民医院妇产科,上海200233 [2]上海交通大学医学院附属国际和平妇幼保健院,上海200030

出　　处：《现代妇产科进展》2014年第11期877-881,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金青年项目(No:81100474)

摘　　要：目的:研究原因不明复发性流产(URSA)患者蜕膜中树突细胞(DC)特异性细胞间黏附分子-3结合非整合素因子(DC-SIGN)和甘露糖受体(MR)及Toll样受体2(TLR-2)和TLR-4的表达情况。方法:采集URSA患者及正常妊娠人工流产妇女(对照组)的新鲜蜕膜。采用免疫组化和Western blot法检测蜕膜中DC-SIGN、MR、TLR-2和TLR-4蛋白表达,RT-PCR检测蜕膜中DC-SIGN、MR、TLR-2和TLR-4 mRNA表达;流式细胞检测蜕膜中DC表面表达DC-SIGN、MR、TLR-2和TLR-4的水平。结果:与对照组比较,URSA组蜕膜中及DC表面的TLR-2和TLR-4表达水平明显升高(P<0.05,P<0.01),DCSIGN表达水平明显降低(P<0.01)。两组的MR表达水平比较,差异无统计学意义(P>0.05)。结论:相对于正常妊娠妇女,URSA患者蜕膜中及树突细胞表面的C型凝集素受体(CLRs)弱势表达,而TLRs优势表达。CLRs与TLRs之间的免疫平衡失调可能是URSA发病机制中的重要因素之一。Objective：To investigate the expression of C-typed lectin receptors （CLRs） of DC-SIGN and MR and Toll-like receptors （TLRs） of TLR-2 and TLR-4 in decidua of unex-plained recurrent spontaneous abortion（ URSA） patients. Methods：The decidua tissues were obtained from URSA patients （URSA group,n =20） and normal pregnant women （NP group,n= 20）. The expressions of DCSIGN,MR,TLR-2 and TLR-4 protein in decidua were detected by immunohistochemistry and Western blot. The levels of DCSIGN,MR,TLR-2 and TLR-4 mRNA in decidua were detected by RT-PCR. The expressions of DCSIGN,MR,TLR-2 and TLR-4 mol-ecule on DC were detected by flow cytometry. Results：The expressions of TLR-2 and TLR-4 on DC and in decidua in URSA group were higher than that in NP group （P〈0. 05 and P〈0. 01 respectively）. The expression of DC-SIGN in URSA group was significantly lower than that in NP group （P〈0. 01）. There was no significant difference in the expression of MR between two groups （P〉0. 05）. Conclusion：Compared with normal pregnant women,the weak expression of CLRs and the overexpression of TLRs were observed in decidua in URSA patients. The imbal-ance between CLRs and TLRs may play an important role in the pathogenesis of URSA.

关 键 词：原因不明复发性流产 蜕膜 C 型凝集素受体 TOLL 样受体 

分 类 号：R714.21[医药卫生—妇产科学]