PTEN在糖尿病肾病患者足细胞损伤中的作用  

Role of PTEN in podocyte injury in patients with diabetic nephropathy

作　　者：邢玲玲[1] 傅淑霞[1] 杨林[1] 姚芳[2] 王建荣[1] 于连英[1] 刘青娟[2] 

机构地区：[1]河北医科大学第二医院肾内科,石家庄050000 [2]河北医科大学病理学教研室,石家庄050017

出　　处：《临床与实验病理学杂志》2014年第12期1375-1378,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省教育厅科学研究项目(2011168);河北省卫生厅医学科学研究重点课题(20110081);教育部高等学校博士学科点专项科研基金(新教师类2013132320001)

摘　　要：目的探讨磷酸酰肌醇3激酶/蛋白激酶B(phosphoinositide 3 kinese/protein kinase B,PI3K/Akt)信号通路抑制因子PTEN与糖尿病肾病(diabetic nephropathy,DN)患者足细胞损伤的关系。方法收集30例DN患者和10例健康志愿者的24h尿标本,采用酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测患者尿液中足盂蛋白(podocalyxin,PCX)的水平;肾活检组织进行形态学观察,根据肾小球病变将DN患者分为3组,免疫组化法检测各组肾小球内p-Akt和PTEN的表达。结果 DN患者尿液中PCX的水平明显高于健康对照组,并随肾小球病变加重其水平逐渐上升;p-Akt和PTEN在DN患者肾小球的表达有所上调,但随着肾小球病变的加重其表达逐渐减少;DN患者尿液中PCX水平与PTEN表达呈负相关,与24h尿蛋白呈正相关。结论 PTEN表达下调可能通过改变Akt的活化状态,从而在DN患者足细胞损伤中发挥一定作用。Purpose To investigate the role of PTEN in podocyte injury in patients with diabetic nephropathy （ DN） . Methods Uri-nary samples from 30 patients with DN and 10 healthy volunteers were collected to detect the level of PCX by ELISA. Renal biopsies were reviewed to observe the morphological changes. All patients with DN were divided into three groups by glomerular lesion. The ex-pression of p-Akt and PTEN in glomeruli was detected by immunohistochemistry. Results The levels of PCX in the urine were signifi-cantly higher in patients with DN compared with those in healthy volunteers, and gradually increased along with glomerular lesion aggra-vating. The expression of p-Akt and PTEN increased in patients with DN compared with healthy volunteers. Although the expression of p-Akt and PTEN decreased with the aggravation of glomerular lesion, they were still higher than that in volunteers. There were obvious-ly positive correlation between the level of PCX and 24-h urinary protein and negative correlation between the level of PCX and the ex-pression level of p-Akt and PTEN. Conclusion PTEN down-regulation may be associated with podocyte injury in DN, which may be associated with the phosphorylation of Akt.

关 键 词：糖尿病肾病 足细胞 足盂蛋白 

分 类 号：R587.1[医药卫生—内分泌]