人白介素-1β水平与人牙周膜细胞OPG表达关系  

Relationship between the level of rh IL-1β and osteoprotegerin expression in human periodontal ligament cells

作　　者：张侬[1] 谢妮[2] 付云[3] 舒睿[1] 杨雅[1] 

机构地区：[1]深圳市龙岗区妇幼保健院口腔科,广东深圳518172 [2]深圳大学第一附属医院中心实验室,广东深圳518035 [3]中山大学光华口腔医学院牙周科,广东广州510055

出　　处：《临床口腔医学杂志》2014年第12期716-718,共3页Journal of Clinical Stomatology

摘　　要：目的:检测不同浓度重组人白介素-1β(recombinant human interleukin-1β,rh IL-1β)对体外培养人牙周膜细胞(human periodontal ligament cells,HPDLCs)表达骨保护因子(osteoprotegerin,OPG)的影响,研究rh IL-1β水平对牙周膜细胞骨向分化的作用。方法:正畸需要而拨除的健康前磨牙牙周膜,体外传代培养HPDLCs;ELISA法和RT-PCR法测定不同浓度rh IL-1β(0、5、10、15μg/L)下HPDLCs的OPG分泌量及m RNA表达。结果:ELISA和RT-PCR法检测结果显示,5、10、15μg/L rh IL-1β作用于HPDLCs均会下调其OPG蛋白分泌和m RNA表达。同一时间点与0μg/L对照组比较,5、10、15μg/L组OPG蛋白分泌和m RNA表达依此下调,差异有统计学意义(P<0.05)。结论:rh IL-1β水平升高会增强对HPDLCs的OPG表达抑制,对牙槽骨健康产生不利影响。Objective：To determine the effects of rhIL-1βon osteoprotegerin expression of cultured human periodon-tal ligament cells （HPDLCs） in vitro,and reach the role of rhIL-1βon the HPDLCs during their osteogenic differentiation. Method：HPDLCs were derived from periodontal membrane of healthy premolars that were extracted for orthodontics and cultured in vitro and secretion levels of the OPG mRNA expression were determined by ELISA and RT-PCR methods under the effect of rhIL-1βat different concentrations （0、5、10、15μg/L）. Result：ELISA and RT-PCR assay showed that rhIL-1βact on HPDLCs reduced the OPG protein secretion and mRNA expression in the 5、10、15 μg/L groups,with negative positively correlated with concentrations. As compared with 0 μg/L control group at the same time,the difference was sig-nificant（P〈0.05） in the 5、10、15μg/L groups. Conclusion：rhIL-1βcan inhibition the OPG expression in HPDLCs at ex-perimental concentration and play an adverse role in the alveolar bone reconstruction.

关 键 词：牙周膜成纤维细胞 重组人白介素-1β 细胞培养 骨保护因子 

分 类 号：Q786[生物学—分子生物学]