卵巢低反应患者抗苗勒管激素与干细胞生长因子表达及其相关性研究  

The Correlation between Anti-M ullerian Hormone and Stem Cell Factor in Patients with Low Ovarian Response

作　　者：王飞苗[1] 胡蓉[1] 李永丽[1] 吴昕[1] 李彩艳[1] 马会明[2] 

机构地区：[1]宁夏医科大学总医院生殖医学中心,宁夏银川750004 [2]省部共建教育部生育力保持重点实验室,宁夏银川750004

出　　处：《实用妇产科杂志》2014年第12期913-917,共5页Journal of Practical Obstetrics and Gynecology

基　　金：2012年国家自然科学基金(编号:81260109);2012年宁夏医科大学校级项目(编号:XQ2012007);2011年宁夏自治区科技攻关项目(编号:KF2010-49)

摘　　要：目的:研究卵巢低反应患者抗苗勒管激素(AMH)和干细胞生长因子(SCF)的表达情况及二者的相关性。方法:选择年龄均小于35岁行体外受精-胚胎移植或卵细胞浆内单精子注射的卵巢低反应患者132例(卵巢低反应组)和正常对照组91例。采用ELISA检测两组患者血清和卵泡液中AMH蛋白和SCF蛋白的表达。分离并培养颗粒细胞,采用RT-PCR检测两组患者颗粒细胞中AMH mRNA和SCF mRNA表达相对灰度值,并对卵巢低反应患者颗粒细胞中两者表达进行相关性分析。结果:1与正常对照组比较,卵巢低反应组血清AMH蛋白表达(2.21±1.11 ng/ml vs 10.49±3.29 ng/ml)和卵泡液AMH蛋白表达(4.21±0.73 ng/ml vs 5.42±0.64 ng/ml)均显著降低,差异均有统计学意义(P<0.01;P<0.05)。2卵巢低反应组颗粒细胞中AMH mRNA表达相对灰度值较正常对照组显著降低(4.10±0.80 vs 6.20±1.40,P<0.01),而SCF mRNA表达相对灰度值较正常对照组明显增加(4.69±1.12 vs 3.10±1.40,P<0.05)。3卵巢低反应组颗粒细胞中AMH mRNA和SCF mRNA的表达呈显著负相关(r=-0.56,P<0.05)。结论:AMH的表达水平与卵巢反应性有关,卵巢低反应患者颗粒细胞中AMH表达显著降低,并与SCF的表达负相关,二者可能存在调控关系。Objective： To study the expressions of anti-mullerian hormone （AMH）and stem cell factor （SCF） and correlation between the two factors in patients with low ovarian response. Methoda：132 patients with low ovarian response （ low ovarian response group） and 91 control cases （ control group） all below 35 years old who experience in vitro fertilization and embryo transfer（IVF-ET） were recruited in the study, the level of AMH and SCF protein in serum and follicular fluid were examined by ELISA;the relative mRNA expression in granulose cells was detected by RT-PCR. The relationship between the expressions of AMH and SCF was analyzed by Pearson＇s correlation. Results：①Compared to control group,the expression of AMH had significantly decreased both in serum （2.21 ± 1.11 ng,/ml vs 10. 49 ± 3.29 ng/ml, P 〈 0. 01 ）and follicular fluid（4.21 ± 0.73 ng/ml vs 5.42 ± 0.64 ng/ml, P 〈 0. 05） in study group. ②Compared to control group, the relative gray-scale value of AMH m RNA expression was decreased （4. 10 ± 0. 80 vs 6.20 ± 1.40, P 〈0. 01 ）, but the relative gray-scale value of SCF mRNA expression was increased（4. 69 ± 1.12 vs 3. 10 ± 1.40, P 〈 0.05 ） in granulose cells in low ovarian response group. ③The expressions of AMH and SCF showed a significant negative correlation in granulose cells in the patients with low ovarian response（ r = -0.56, P 〈0. 05）. Conclusions：The expression of AMH is related with the ovarian response, which decreases in granulose cells of patients with low ovarian response, moreover AMH and SCF show a negative correlation which reveals AMH may regulate SCF＇s expression in human ranulose cells.

关 键 词：抗苗勒管激素 干细胞因子 颗粒细胞 卵巢低反应 

分 类 号：R711.75[医药卫生—妇产科学]