正交试验优化超临界CO_2萃取法提取黄芪中黄芪甲苷工艺  

Extraction of astragaloside Ⅳ in astragalus by supercritical CO_2 extraction technique optimized by orthogonal test

作　　者：纪莎[1] 胡雯玲[1] 何丹鸿[2] 袁曦[2] 黄为榕 

机构地区：[1]福建中医药大学附属第二人民医院,福建福州350003 [2]福建医科大学附属第一医院,福建福州350005 [3]福建医科大学药学院,福建福州350005

出　　处：《中国医院药学杂志》2015年第1期65-67,共3页Chinese Journal of Hospital Pharmacy

基　　金：福建省科学技术厅战略性新兴产业资助项目(编号:2013Y0049)

摘　　要：目的:优化超临界CO2萃取法提取黄芪中黄芪甲苷工艺。方法:用超临界CO2萃取法提取黄芪中黄芪甲苷,以萃取温度、萃取压力、萃取时间、萃取剂流速为考察因素,黄芪甲苷得率为考察指标,运用正交试验优化提取的最佳工艺条件,同时用方差分析对其进行评价。结果:实验结果表明,超临界CO2萃取法提取黄芪中黄芪甲苷的最佳工艺条件为:萃取温度为45℃、萃取压力为45 MPa、萃取时间为2 h、萃取剂流速为20 L·h-1。结论:本提取工艺条件可行,为黄芪中黄芪甲苷的提取方法的及黄芪甲苷的研究提供科学依据。OBJECTIVE To optimize supercritical CO2 extraction method to extract astragaloside Ⅳ in Astragalus. METHODS In this experiment, the supercritical CO2 extraction method was used to extract astragaloside Ⅳ in Astragalus, with extraction temperature, pressure, time, solvent flow rate as the inspection factors, astragaloside Ⅳ yield as inspection index, orthogonal test was used to optimize the extraction conditions, and analysis of variance was used to evaluate the performance. RESULTS The experimental results showed the optimal conditions of supercritical CO2 extraction method for astragaloside Ⅳ in astragalus： extraction temperature 45 ℃, pressure 45 MPa, duration 2 h, flow rate of extractant 20 L·h^-1. CONCLUSION This extraction process is feasible, and provides scientific basis for extraction method of astragaloside Ⅳ in Astragalus, and the study on astragaloside Ⅳ.

关 键 词：黄芪 黄芪甲苷 超临界萃取法 正交试验 

分 类 号：R944[医药卫生—药剂学]