Cd暴露对南方鲇氧化应激及脑胆碱酯酶活性的影响  

Effects of Cadmium Exposure on Oxidative Stress and Activity of Acetylcholin Esterase(AChE)in Southern Catfish(Silurus meridionalis Chen)

作　　者：张玄可 梁敏[1] 程卫东[1] 袁伦强[1] 

机构地区：[1]西南大学淡水鱼类资源与生殖发育教育部重点实验室,重庆400715

出　　处：《重庆师范大学学报（自然科学版）》2015年第1期41-45,共5页Journal of Chongqing Normal University:Natural Science

基　　金：国家重点基础研究发展计划(No.2012CB723205);重庆市科委重点实验室专项经费(2013)

摘　　要：采用水体染毒法研究了南方鲇(Silurus meridionalis Chen)幼鱼Cd暴露0、7、14d时肝脏中总抗氧化能力(TAOC)、金属硫蛋白(MT)含量、丙二醛(MDA)含量的变化以及血液中血红蛋白(Hb)含量、脑中胆碱酯酶(AChE)活性的变化。实验设置1个对照组和4个不同Cd含量(0.015、0.06、0.24、0.96 mg·L-1)的处理组。结果显示,Hb含量和AChE活性先显著性降低(p<0.05)然后恢复到正常水平;T-AOC水平和MT含量的变化为随Cd含量升高和暴露时间延长而显著性升高和增加(p<0.05);MDA含量在暴露14d时显著性降低(p<0.05)。研究提示,AChE活性和Hb含量先受到Cd暴露影响,而南方鲇通过肝脏增加合成MT、提高T-AOC水平来对抗Cd的毒害;较长时间的Cd暴露使肝脏MDA含量降低,同时合成MT的能力和T-AOC水平明显升高,表现出"毒物兴奋效应";南方鲇可通过调节体内生理功能使之应对环境中一定范围的Cd污染胁迫。We examined the effects of Cadmium （Cd） on malondialdehyde （MDA） content, the total antioxidant capacity （T-AOC）, metallothionein （MT） content, hemoglobin （Hb） content and activity of acetyleholin esterase （ACHE） in juvenile southern catfish （Silurus meridionalis Chen）, after exposure to the waterborne Cd for 0, 7 and 14 days. The concentrations of Cd was designed as 0（the control）, 0. 015, 0.06, 0.24 and 0.96 mg· L^-1. The results showed that Hb content and AChE activity decreased with increasing Cd at 7 days （p〈0.05）, but there was no significant difference about Hb content and AChE activity between Cd treatments and the control at 14 days （p〉0.05）. T-AOC and MT content in liver increased with increasing Cd at 7days, T-AOC progressively increased with increasing Cd at 14 days （p〈0.05）. The hepatic MDA content in Cd treatments decreased with increasing Cd at 14 days （p〈0.05）. It suggests that the effects of Cd exposure on southern catfish was reflected in the AChE activity and Hb content at 7 days,however, southern catfish increased T-AOC and MT synthesis against cadmium poisoning. The effects of Cd exposure was reflected in the MDA decrease accompanied the significantly increase of T-AOC and MT synthesis; it was characterized by ＂hormesis＂. Southern catfish was able to adjust its physiological functions and reduce the damage caused by the certain concentrations of Cd pollution stress in environment.

关 键 词：Cd 南方鲇(Silurus MERIDIONALIS Chen) 总抗氧化能力(T-AOC) 丙二醛(MDA) 金属硫蛋白(MT) 血红蛋白(Hb) 胆碱酯酶(AChE) 

分 类 号：Q494[生物学—生理学]