氯离子对氧化亚铁硫杆菌和氧化亚铁钩端微螺菌活性的影响  

Effect of Chloride Ions on Activity of Acidimicrobium Ferrooxidans and Leptospirillum Ferriphilum Ferrous

作　　者：郭勤[1] 韩文艳[1] 李江[1] 刘亚洁[1] 史维浚[1] 郑福存 

机构地区：[1]东华理工大学省部共建核资源与环境教育部重点实验室,南昌330013

出　　处：《有色金属（冶炼部分）》2015年第1期42-45,53,共5页Nonferrous Metals（Extractive Metallurgy)

基　　金：国家高技术研究发展计划(863计划)项目(2012AA061504);科技部国际合作项目(2011DFR60830)

摘　　要：采用连续稀释法分别从江西和广东某铀矿生物冶金现场筛选得到两株亚铁氧化菌。16srDNA鉴定结果表明,细菌分别为氧化亚铁硫杆菌（Acidimicrobium ferrooxidans）和氧化亚铁钩端微螺菌（Leptospirillum ferriphilum）。在氯离子浓度在1g/L以内,对细菌亚铁氧化活性基本没有影响;1-2g/L铁氧化活性有所降低,但是细菌通过调节依然能够适应并维持比较高的活性;氯离子浓度大于2g/L后亚铁氧化活性急速下降,当氯离子浓度大于3g/L后细菌生长受到抑制,亚铁氧化活性很低。Two acidicbacillus strains were isolated from samples of uranium biological heap leaching ore in Jiangxi and Guangdong province by successive diluting method.16 srRNA gene sequence analysis confirms that one bacteria is Acidimicrobium ferrooxidans and the other is Leptospirillum ferriphilum.Concentration of chlorine ion of 1g/L below has no effect on ferroxidase activity.Activity weakens with concentration of chloride ion in 1-2g/L,but proper adjustment can enable bacterium to adapt and maintain acceptable activity.However,when chloride ion concentration is 2 g/L above,ferroxidase activity weakens rapidly and growth of bacteria is even inhibited with lower ferroxidase activity when concentration of chloride ion is 3g/L above.

关 键 词：氧化亚铁硫杆菌 氧化亚铁钩端螺旋菌 氯离子 亚铁氧化活性 

分 类 号：TL212.12[核科学技术—核燃料循环与材料]