银杏叶提取物对肝纤维化大鼠肝脏内质网应激相关c-jun氨基末端激酶通路的影响  

Effects of ginkgo biloba extract on the hepatic endoplasmic reticulum stress-related JNK pathway in rats with hepatic fibrosis

作　　者：叶蕾[1] 余亚平[1] 严茂祥[1] 李剑霜[1] 陈芝芸[1] 

机构地区：[1]浙江省中医院,杭州310006

出　　处：《中华中医药杂志》2015年第1期293-295,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省教育厅科研项目(No.Y201223352)~~

摘　　要：目的:观察银杏叶提取物(Gb E)对肝纤维化大鼠肝组织内质网应激相关c-jun氨基末端激酶(JNK)通路的影响,探讨Gb E抗肝纤维的作用机制。方法:采用40%的CCl4皮下注射(3m L·kg-1·d-1,2次/周,首剂加倍),连续6周诱导大鼠肝纤维化模型,同时分别以低、中、高剂量(50、100、200m L·kg-1·d-1)Gb E灌胃干预6周,空腹取肝组织,Real-time PCR法检测JNK m RNA表达,Western blot技术检测大鼠肝组织中JNK通路关键分子凋亡信号调节激酶(ASK)及JNK的总蛋白及其磷酸化表达。结果:肝组织JNK m RNA表达及ASK、JNK总蛋白活化水平较正常组明显增强(P<0.05,P<0.01);Gb E低、中、高剂量组大鼠肝组织JNK m RNA表达及ASK、JNK总蛋白活化水平较模型组不同程度下降(P<0.05,P<0.01)。结论:CCl4诱导的肝纤维化大鼠肝脏内质网应激相关的JNK通路被激活,Gb E可通过调节肝纤维化大鼠JNK通路防止肝纤维化的进展。Objective： By observation of the role of ginkgo biloba extract （GbE） on the hepatic endoplasmic reticulum （ER） stress-related JNK pathway in rats with hepatic fibrosis, to explore anti hepatic fibrosis mechanism of GbE. Methods： The normal rats were received the subcutaneous injection of 40% CC14 for establishing hepatic fibrosis model, at 3mL·kg^-1·d^-1, twice a week, lasting for 6 weeks. Meanwhile, all the rats were gavaged with different doses of GbE （50, 100 and 200mg·kg^-1·d^-1）. After 6 weeks of administration, hepatic tissue was obtained. The expression of hepatic JNK mRNA was detected with Real-time PCR. The expressions of ASK protein, the key molecule in JNK pathway and the expression of total JNK protein and its phosphorylation protein were detected with Western blot. Results： The expression of hepatic JNK mRNA and the expression of hepatic ASK protein and JNK protein in the model rat group were higher than those in the normal group （P〈0.05, P〈0.01）, while in different doses of GbE groups were lower than that in the model group （P〈0.05, P〈0.01）. Conclusion： The hepatic endoplasmic reticulum （ER） stress-related JNK pathway can be activated by CC14 inducing hepatic fibrosis. GbE could prevent the development of hepatic fibrosis by regulating the JNK pathway.

关 键 词：肝纤维化 内质网 c-jun氨基末端激酶通路 银杏叶提取物 内质网应激 

分 类 号：R285.5[医药卫生—中药学]