人参皂苷含药血清对成骨细胞增殖和活性的影响  

Effects of serum from Ginsenoside saponins on the proliferation and differentiation of osteoblasts

作　　者：方秀统[1] 李洋[2] 于方[1] 陈国强[1] 俞猛[1] 徐华丽[3] 

机构地区：[1]首都医科大学附属世纪坛医院骨科,100038 [2]吉林大学第一医院胸外科 [3]吉林大学药学院药理教研室

出　　处：《中华医学杂志》2014年第45期3596-3598,共3页National Medical Journal of China

摘　　要：目的探讨人参皂苷（GSS）含药血清对成骨细胞（OB）增殖、分化和矿化能力的影响。方法源于大鼠颅盖骨的原代OB中加入不同浓度的人参皂苷含药血清进行干预。M1rr法观察人参皂苷含药血清对OB增殖的影响，用硝基苯磷酸盐（PNPP）偶氮法观察人参皂苷含药血清对OB碱性磷酸酶（ALP）活性的影响，茜素红（ARS）进行矿化结节染色并计算面积以观察人参皂苷含药血清对OB矿化能力的影响。结果人参皂苷含药血清可使体外培养OB的增殖率明显提高[（2．1990±0．2778）比（1．2165±0．1417），P〈0．01）]，并可明显促进0B的矿化能力[（23．7±5．1）mm^2／孔比（9．0±5．1）mm^2／孔，P〈0．05]，其对OB的ALP活性影响作用也同样明显[（21．24±3．2）U／mg比（10．2±2．9）U／mg，P〈0．01]。结论人参皂苷含药血清可促进0B的增殖能力和矿化能力。Objective To explore the effects of serum from ginsenoside saponins （GSS） on the proliferation, differentiation and mineralization in osteoblasts （OB）. Methods Serum from GSS with different doses were fed to calvaria bone specimens from rats. The proliferation of osteoblasts and the alkaline phosphatase （ALP） activity of OB were analyzed. After alizarin red S staining, the area of calcium nodule was calculated to observe the result of serum from GSS on OB mineralization. Results Serum from GSS had notable effects on the proliferation of in vitro cultured OB （P 〈 0.01 ） and remarkably stimulated the mineralization of OB （P 〈0. 05） while its effect on the ALP activity of OB was also marked （P 〈0. 01 ）. Conclusion Serum from GSS can promote the proliferation and mineralization ability of OB and sianificantlv accelerate the formation of OB.

关 键 词：骨质疏松 人参皂苷类 成骨细胞 含药血清 

分 类 号：R285[医药卫生—中药学]