小麦八氢番茄红素合成酶基因的克隆和序列分析  

Cloning and Sequence Analysis of Wheat Phytoene Synthase Gene

作　　者：刘慧娟[1,2] 冯志国[1,2] 董瑶[1] 汤贞贞 何光源[2] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000 [2]华中科技大学分子生物物理教育部重点实验室,湖北武汉430074

出　　处：《河南农业科学》2014年第12期15-18,33,共5页Journal of Henan Agricultural Sciences

基　　金：国家高技术研究发展计划项目(2002AA224031)

摘　　要：为了探讨小麦中类胡萝卜素的合成机制,通过RACE方法（rapid amplification of cDNA ends）从小麦中克隆到1个新的八氢番茄红素合成酶（PSY）基因,用DNAMAN软件对PSY基因序列进行分析并构建了系统进化树。结果表明,该基因cDNA序列长为1 151bp,编码304个氨基酸,分子量为33.9kD。小麦的PSY基因与其他植物PSY基因高度同源,相似性为60%~92%,其中与水稻、玉米中PSY2的同源性高达90%以上,系统进化树分析表明,PSY基因在植物进化上是高度保守的。软件分析表明,克隆的PSY基因属于PSY2群,将该基因命名为TaPSY2。In order to understand the synthesis mechanism of carotenoids in wheat,a new phytoene synthase（PSY）gene was cloned from wheat by RACE.The PSY gene was analyzed and a phylogenetic tree was constructed by DNAMAN.The results showed that the gene cDNA was1 151 bp in length and encoded a polypeptide of 304 animo acids with a calculated molecular mass of 33.9kD.A BLAST search in GenBank showed that wheat PSY was highly similar to that in other plants（60%-92%）.Especially,the identity of the wheat PSY gene with PSY2 genes in rice and maize was over 90%.Phylogenic analysis showed that PSY was highly conservative in evolution of plants.Motif analysis showed that the cloned gene belonged to PSY2 group,which was designated as TaPSY2.

关 键 词：八氢番茄红素合成酶基因 小麦 cDNA末端快速克隆 序列分析 

分 类 号：S512.1[农业科学—作物学]