谷子、玉米重复基因间基因置换的系统发育分析  

Phylogenetic Analysis of Gene Conversion between Duplicated Genes in Setria italica and Zea mays

作　　者：王金朋[1] 聂林曼 王振怡[1] 马雪莲[1] 张琼[1] 

机构地区：[1]河北联合大学生命科学学院基因组学与计算生物学研究中心,河北唐山063009

出　　处：《河南农业科学》2014年第12期34-39,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31100913);河北联合大学科学研究基金项目(z201230;z201235);河北联合大学大学生创新性实验计划项目(X2013044)

摘　　要：以谷子和玉米为研究对象,利用系统发育分析和比较基因组学推断了重复基因间可能的基因置换,旨在对其基因组中由全基因组加倍产生的重复基因的相互作用机制进行研究。结果表明,谷子中有192对(18.9%)、玉米中有121对(11.9%)的重复基因在其进化过程中发生了基因置换,且重复基因对之间的置换常常是以部分基因置换为主,谷子发生置换的基因对中有88.4%、玉米中有76.5%是部分基因置换;基于动态规划和系统发育相结合的方法,确定了谷子、玉米多个重复基因对间发生过不止1个片段的基因置换;对基因置换与重复基因在染色体上物理位置的相关性分析发现,基因置换与基因在染色体上的位置显著相关,对不同染色体区域重复基因发生置换的频率进行统计分析表明,靠近染色体末端的重复基因对更易发生置换。Setaria italica and Zea mays were taken as the materials to study the interaction mechanism of duplicate genes produced by whole genome doubling,and to infer the possible gene conversion between duplicate genes by means of the phylogenetic analysis and comparative genomics.The result showed that there were 192 pairs of duplicate genes in Setaria italica and121 pairs in Zea mays that came into gene conversion in the evolution,most of which were partial conversion that accounted for 88.4% in Setaria italica and 76.5% in Zea mays.Based on the method combining dynamic programming and phylogenetic development,we confirmed that one or more fragments of gene conversion occurred between the multiple duplicate gene pairs in Setaria italica and Zea mays.The correlation analysis of gene conversion and duplicated genes in the physical location of chromosome showed that there was significant correlation between the gene conversion and the gene location on chromosome.The frequency of gene conversion in different chromosomal regions was analyzed statistically,which showed that the duplicate genes close to the terminal of chromosome were more prone to convert.

关 键 词：谷子 玉米 重复基因 基因置换 系统发育分析 

分 类 号：S513[农业科学—作物学] S515