马铃薯体细胞杂种后代青枯病抗性鉴定及分子标记检测  

Evaluation of Resistance and Associated Molecular Markers of Bacterial Wilt of Potato Somatic Hybrid Offspring

作　　者：李朋[1] 蔡兴奎[1] 陈琳[1] 柳俊[1] 

机构地区：[1]华中农业大学生命科学技术学院,华中农业大学园艺植物生物学教育部重点实验室,国家蔬菜改良中心华中分中心,湖北武汉430070

出　　处：《中国马铃薯》2014年第6期321-327,共7页Chinese Potato Journal

基　　金：教育部创新团队(IRT13065);现代农业产业技术体系建设专项资金(CARS-10-P06)

摘　　要：马铃薯(Solanum tuberosum L.)青枯病是由茄科雷尔氏菌(Ralstonia solanacearum)引起的一种毁灭性的细菌性土传病害。马铃薯青枯病抗性资源主要存在于一些野生种中,体细胞杂交是创制马铃薯青枯病抗性资源的一种有效途径。本研究以具有对青枯病抗性的体细胞杂种与栽培种杂交产生的100个后代为材料,对其进行青枯病抗性评价,旨在筛选可供育种利用的青枯病抗性资源。青枯病抗性鉴定结果表明,在试管苗组培鉴定中100个杂交后代共有6个基因型表型抗青枯病,温室钵栽接种鉴定有8个基因型表现为抗病,在两种接种鉴定中均表现为抗病的有3个基因型(07SF.3-79、07SF.6-8和07SF.6-5)。选用本实验室前期筛选的与青枯病抗性相关的4对SSR标记引物(STI0051、STI0054、STI0056、STI0057),对100个基因型进行分子标记检测,结果显示,有3个标记(STI0051.180、STI0054.180、STI0056.205)可以明确鉴定抗感基因型,它们表现为抗病稳定的3个基因型的标记位点与抗病对照的带型一致,而在感病对照中缺失,与表型鉴定结果吻合,表明筛选的SSR标记可以用于具有S.chacoense遗传背景材料的青枯病抗性辅助选择。Ralstonia solanacearum, the causal pathogen of potato （Solanum tuberosum L.） bacterial wilt, is soil-borne and destructive for potato production. Somatic hybridization between S. tuberosum and wild relatives is an effective way to obtain germplasm possessing resistance to R. solanacearum. Evaluation of resistance to R. solanacearum on 100 progenies derived from somatic hybridization between S. tuberosum and wild relatives were conducted in this study to screen for germplasms that possess resistance to R. solanacearum and are relevant to breeding. The results showed that six clones exhibited resistance to the pathogen in vitro inoculation and eight clones performed resistance to the pathogen by the inoculation with greenhouse grown plants among the 100 progenies. Noticeably, three resistant clones （07SF.3-79, 07SF.6-8 and 07SF.6-5） were consistent for resistance level in the two tests. Four SSRs （STI0051, STI0054, STI0056 and STI0057） previously identified were used to test al of the 100 clones mentioned above. The results demonstrated that three markers （STI0051.180, STI0054.180 and STI0056.205） could differentiate resistant genotype from susceptible ones. The three clones considered resistant in the two inoculations amplified the same bands as resistant control while they were absent in susceptible control. These were in accordance with the disease phenotyping and suggest an application potential of these SSRs in marker-assistant selection of bacterial wilt resistance introgressed from S. chacoense.

关 键 词：马铃薯 体细胞杂种 青枯病 抗性鉴定 分子标记 

分 类 号：S532[农业科学—作物学]