O-(β-hydroxyethyl)rutosides determination by micellar flow injection(FI)-spectrofluorimetry  

作　　者：Cecilia Mariana Peralta Liliana Patricia Fernndez 

机构地区：[1]Instituto de Químrica de San Luis (INQUISAL-CONICET), 5700 San Luis, Argentina [2]rea de Qui mica Fisica, Facultacl de Química,Bioquímica y Farmacia, Chacabuco y Pedemera,Universidaci Nacional de San Luis, 5700 San Luis, Argentina [3]reci de Qufmica Analítica, Facultacl de Química, Bioquímica y Farmacia, Chacabuco y Pedemera,Universidad Nacional de San Luis, 5700 San Luis, Argentina

出　　处：《Journal of Pharmaceutical Analysis》2014年第6期368-373,共6页药物分析学报（英文版）

基　　金：the National University of San Luis(Project 22/Q228);INQUISAL-CONICET(Instituto de Quimica de San Luis-Consejo Nacional de Investigaciones Cientificas y Tecnicas, PIP-CONICET 11220100100405) for the financial support

摘　　要：A simple,eco-friendly.sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-（β-hydroxyethyl）rutosidcs.The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-（β-hydroxyethyl）rutosides fluorescence enhancement,increasing considerably the sensitivity of detection.All the variables affecting the fluorescence intensity were studied and optimized.The flow rate was 5 mL/min with detection at 450 nm（after excitation at 346 nm）.A linear correlation between drug amount and peak area was established for 0-（β-hydroxyethyl）rulosides in the range of 0.01-200 μg/mL with a detection limit of0.001 μg/mL（s/n = 3）.Validation processes were performed by recovering studies with satisfactory results.The new methodology can be employed for the routine analysis of 0-（P-hydroxyethyl）rutosides in bulks as well as in commercial formulations.A simple,eco-friendly.sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-（β-hydroxyethyl）rutosidcs.The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-（β-hydroxyethyl）rutosides fluorescence enhancement,increasing considerably the sensitivity of detection.All the variables affecting the fluorescence intensity were studied and optimized.The flow rate was 5 mL/min with detection at 450 nm（after excitation at 346 nm）.A linear correlation between drug amount and peak area was established for 0-（β-hydroxyethyl）rulosides in the range of 0.01-200 μg/mL with a detection limit of0.001 μg/mL（s/n = 3）.Validation processes were performed by recovering studies with satisfactory results.The new methodology can be employed for the routine analysis of 0-（P-hydroxyethyl）rutosides in bulks as well as in commercial formulations.

关 键 词：O-（β-hydroxyethyl）rutosides Micellar enhancement Flow injection SPECTROFLUORIMETRY Pharmaceuticals 

分 类 号：R917[医药卫生—药物分析学]