HPLC法同时测定地桃花的槲皮素和山奈酚  

Simultaneous Determination of Quercetin and Kaempferol in Urena lobata by HPLC

作　　者：卫亚丽[1] 吕昱[1] 杨琼[1] 何可群[1] 

机构地区：[1]贵州民族大学化学与环境科学学院,贵州贵阳550025

出　　处：《贵州农业科学》2014年第12期66-69,共4页Guizhou Agricultural Sciences

基　　金：国家社会科学基金项目"白族民间医药文化调查研究"(12XMZ033);贵州省科技厅贵州民族大学联合项目[黔科合J字LKM(2012)04]

摘　　要：为更好地控制地桃花的质量,建立高效液相色谱法同时测定地桃花中槲皮素和山奈酚含量的方法,对地桃花中槲皮素和山奈酚的含量进行测定。结果表明：用C18色谱柱（100mm×4.6mm,5μm）,以甲醇-0.4%磷酸水溶液（45∶55）为流动相,流速0.8mL/min,检测波长360nm,测定出槲皮素和山奈酚的线性范围均为2.500-25.00μg/mL,相关系数r分别为0.999 7和0.999 9,精密度RSD（n=5）分别为0.98%和0.79%,稳定性试验RSD（n=5）分别为1.36%和1.13%,加样回收率RSD（n=5）分别为2.37%和2.10%。该方法适于同时测定地桃花中的槲皮素及山奈酚含量,方法简便可行,重现性好,精密度高,结果可靠。地桃花中槲皮素和山萘酚的最高含量分别为（1.067±0.02）mg/g和（1.232±0.03）mg/g。To better control the quality of U.lobata,an HPLC method was established for simultaneously determining quercetin and kaempferol of U.lobata and the content of quercetin and kaempferol were determined.The column was agilent Eclipse plus C18（100mm×4.6mm,5μm）column.The mobile phase was mixture of methanol and 0.4% phosphoric acid water solution（45∶55）.The flow rate was 0.8mL/min,the wavelength was 360 nm.The results showed that the calibration curves of two flavonoids were both linear in the range of 2.500-25.00（g/mL（r = 0.9997 and 0.9999respectively）.The precisions test RSD were 0.98% and 0.79% respectively.The stability test RSD were 1.36% and1.13%respectively.The RSD were 2.37% and 2.10%respectively.The method was accurate with high sensitivity and suitable for the determination of quercetin and kaempferol in U.lobata.The highest contents of quercetin and kaempferol in U.lobata were respectively（1.067 0.02）mg/g and（1.232 0.03）mg/g.

关 键 词：HPLC 地桃花 槲皮素 山奈酚 

分 类 号：S567.9[农业科学—中草药栽培] R917[农业科学—作物学]