植物病原根结线虫的染色方法研究  

Study on Methods for Staining Root-knot Nematodes Using Different Dyes

作　　者：李勇军[1] 李宏光[2] E.Schliephake H.Budahn 朋金娥[1] 刘春明[2] 张绍松[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,农业部西南作物基因资源与种质创制重点实验室,云南省农业生物技术重点实验室,云南昆明650223 [2]云南省烟草公司红河州公司,云南弥勒652300 [3]Julius Kuhn-Institut, Federal Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, 06484 Quedlinburg, Emst-Baur-Str. 27, Germany [4]Julius Kuhn-Institut,Federal Centre for Cultivated Plants, Institute for Breeding Research on Horticultural and Fruit Crops, 06484 Quedlinburg, Ernst-Banr-Str.26, Germany)

出　　处：《西南农业学报》2014年第6期2405-2412,共8页Southwest China Journal of Agricultural Sciences

基　　金：农业部科教司引进交流处中德农业科技合作项目;云南省科技厅国际合作项目(2002GH05);红河州烟草公司项目(2011HYZ01)

摘　　要：根结线虫是许多农作物的重要病原,在农业生产中常极大地降低农作物的产量和质量。本研究中选用了7种不同染料,分别对侵入寄主根系组织初期的J2幼虫、雌虫产生的卵块团、雌虫尾部会阴花纹、及鉴别J2幼虫的死虫与活虫的染色方法进行了探索,这些方法便于有效了解和识别病原在侵染寄主不同阶段的形态。试验结果表明,溴酚蓝溶液对侵入根系的J2幼虫具有良好的染色效果;对于根系上的卵块团与雌虫的会阴花纹,cochenille red A或phloxine B溶液的染色效果较清晰;用甲基蓝与吖啶橙溶液分别对J2幼虫染色后,可相应地在可见光和荧光显微镜下判别J2幼虫中的死虫。Root-knot nematodes are important pathogens for a great number of crops. Meloidogyne species can significantly reduce yield and quality in agricultural production. In this study,seven different dyes were tested to stain the larvae infecting initially the host roots and the egg masses produced by adult females to describe the perineal pattern of female tail and distinguish alive from dead larvae. These methods should be suitable to recognize the morphology of pathogens in infecting stage. The result showed that the bromophenol blue solution had the best tinting effect to stain the larvae within the root. For staining of egg masses and the perineal pattern around female vulva,cochenille red A or phloxine B solution could be recommended. Died J2 juveniles were distinguished from living larvae by methyl blue or acridine orange solution by microscope under visible or fluorescent light,respectively.

关 键 词：根结线虫 染料 染色 方法 

分 类 号：S436[农业科学—农业昆虫与害虫防治]