锰营养对甘草光合特性和抗氧化酶活性的影响  

Effect of Different Concentration of Mn on Photosynthetic Characteristics and Antioxidase Activities of Glycyrrhiza uralensis Fisch

作　　者：马生军[1,2] 程新宇[1] 谢景[1] 王文全[1,3,4] 侯俊玲[1,4] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193 [4]中药材规范化生产教育部工程研究中心,北京100102

出　　处：《现代中药研究与实践》2014年第6期7-10,共4页Research and Practice on Chinese Medicines

基　　金：国家公益性(中医药)行业科研专项项目(201107009-03);中药材规范化生产技术服务平台(工信部消费(2011)340)

摘　　要：目的探讨不同浓度的锰营养水平对甘草光合特性和抗氧化酶活性的影响。方法以一年生甘草移栽苗为试验材料,采用盆栽蛭石的方法,每周向盆内浇灌不同浓度锰的营养液,锰处理水平分别为0 mg/L、1.81 mg/L、18.1 mg/L和36.2 mg/L,其中1.81 mg/L即正常Hoagland营养液中锰的浓度。采用LI-6400光合仪测定其光合生理指标,色素和抗氧化酶活性的测定则通过植物生理学常规方法进行。结果随着锰处理浓度的增加甘草叶片叶绿素和类胡萝卜素含量逐渐增加,18.1 mg/L处理时均达到最大值,与0 mg/L相比差异显著(P<0.05)。在锰浓度18.1 mg/L处理时净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)达到最大值,而胞间CO2浓度(Ci)在1.81 mg/L处理时达到最大值。对抗氧化酶活性的分析结果表明,随着锰处理浓度的增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)三种抗氧化酶活性均显著增加。结论缺锰会抑制甘草的各项生理功能,适当浓度的锰处理能够显著提高甘草的光合指标和抗氧化酶活性。Objective To investigate the effect of different concentrations of manganese （Mn） on the photosynthetic characteristics and antioxidase activities of Glycyrrhiza uralensis Fisch. Method The transplants of one-year-old G. uralensis were subjected to four concentration of Mn （0,1.81,18. land 10.4 mg/L） culturing in vermiculite and 1.81mg/L Mn was used as the normal concentration in the complete Hoagland nutrition solution. The solution was irrigated to the pots every week. The photosynthetic physiological indicators were measured by LI-6400 photosynthetic instrument. The pigments and antioxidase activities of the leaves were determined by general method in plant physiology. Results The chlorophyll and eartenoid content increased significantly with the increasing of Mn concentrations, under 18.1 mg/L Mn treatment reached the highest compared with other Mntreatment（P 〈 0.05）. The photosynthetic indicators of Pn, Gs, Tr reached the highest under 18.1 mg/L Mn treatmen, however, did Ci under 1.81 mg/L Mn treatmen. The results of analysis of antioxidase activities showed that all of superoxide dismutas （SOD）, peroxidase （POD）, catalase （CAT） increased significantly with the increasing of Mn treatment. Conclusions The deficiency of Mn could inhibit the physiological function of G. uralensis in all aspects. Meanwhile, appropriate concentration of Mn treatment could significantly increase the photosynthesis and the activity of antioxidant enzymes of G. uralensis.

关 键 词：锰 甘草 光合特性 抗氧化酶活性 

分 类 号：S567[农业科学—中草药栽培]