波叶青牛胆提取物对糖尿病肾病小鼠药效及急性毒性  

Effect of Tinospora crispa extract on diabetic nephropathy mice and their acute toxicology

作　　者：马珍[1] 黄丹民[1] 张卫华[1] 孙艳[1] 陈钧[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013

出　　处：《中成药》2015年第1期6-12,共7页Chinese Traditional Patent Medicine

摘　　要：目的研究波叶青牛胆的乙醇提取物和不同极性溶剂萃取部位对Ⅱ型糖尿病肾病小鼠的疗效以及急性毒性。方法 100只采用高脂饮食联合小剂量链脲佐菌素(STZ)复制Ⅱ型糖尿病肾病成功的ICR小鼠分为模型组,乙醇提取物低、中、高剂量组,三氯甲烷部位组,乙酸乙酯部位组,正丁醇部位组,水溶性部位组,以及卡托普利组和消渴丸阳性药组,并设正常对照组。给药6周末测定各组小鼠血清中总胆固醇、尿素氮、肌酐、胱抑素C水平,PAS染色观察肾脏病理变化。结果乙醇提取物的LD50为4 887 mg/kg。与模型组相比,乙醇提取物高剂量组和正丁醇部位组能显著改善糖尿病肾病小鼠各血清生化指标水平,缓解肾小球肥大,肾小球基底膜增厚及系膜扩张等病理改变。结论波叶青牛胆属于低毒类中草药,在其安全剂量范围内可以降低糖尿病肾病小鼠的血糖、总胆固醇,改善肾功能状态。AIM To observe the effect of Tinospora crispa ethanol extract（ TCEE） and their solvent extracts on ICR mice with diabetic nephropathy（ DN） and their acute toxicities. METHOD LD50 of TCEE was determined by modified Kaber method. One hundred mice with DN induced by injection of STZ and fed high fat diet were divided equally into vehicle-treated group,the low,middle and high dose TCEE groups,Et OAc fraction group,Bu OH fraction group,water soluble fraction group,Captopril group and Xiaoke Pills group,and setting the control group（ n = 10）. At the end of 6thweek,the effects of T. crispa on serum biochemical indexes（ BUN,Crea,TC,and Cys-C） and the PAS staining histomorphology of kidney tissues were evaluated. RESULTS LD50 of TCEE was 4 887 mg / kg. The levels of biochemical indexes of mice with diabetic nephropathy were significantly reduced with an improved renal function in TCEE and Bu OH fraction group. PAS staining showed that the symptoms,including glomerular hypertrophy,thickening of glomerular basement membrance,and mesangial expansion,were significantly improved,especially in high dose TCEE group and Bu OH fraction group. CONCLUSION T.crispa is a kind of low toxicity herb and it can reduce the level of blood glucose,total cholesterol and improve the renal function in DN mice in the safety dosage.

关 键 词：波叶青牛胆 半数致死量 糖尿病肾病 小鼠 

分 类 号：R285.5[医药卫生—中药学]