马钱子组分对人HaCaT细胞毒性作用的比较  

Cytotoxicity of components from seeds of Strychnos nux-vomica to human HaCaT keratinocytes

作　　者：陈海波[1] 马凤森[1,2] 方剑乔[2] 方芳[2] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014 [2]浙江中医药大学附属第三医院,浙江杭州310009

出　　处：《中成药》2015年第1期16-21,共6页Chinese Traditional Patent Medicine

基　　金：浙江省重大科技专项重点社会发展项目(2010C13014);浙江省自然科学基金项目(Y2111194)

摘　　要：目的研究马钱子生物碱及其不同组分对人永生化表皮细胞系Ha Ca T细胞的毒性作用。方法体外培养Ha Ca T细胞,加入相同质量浓度的马钱子碱、士的宁、马钱子碱与士的宁的混合物,和马钱子总生物碱作用于细胞24 h后,采用MTT比色法测定不同生物碱的细胞增殖抑制作用;流式细胞仪检测对Ha Ca T细胞的凋亡,比较马钱子生物碱不同组分对Ha Ca T细胞的毒性作用。结果士的宁对Ha Ca T细胞的抑制率和细胞凋亡率大于马钱子碱;马钱子碱和士的宁混合物组对Ha Ca T细胞的抑制率和细胞凋亡率大于马钱子总生物碱。结论马钱子生物碱组分均对Ha Ca T细胞存在细胞毒作用,呈剂量依赖关系,但马钱子碱的细胞毒性明显小于士的宁。AIM To study the cytotoxicity of alkaloids from Strychni Semen and its different components on Ha Ca T cell,an immortalized human epidermal cell line. METHODS After in vitro Ha Ca T cells being cultured co-cultured with same concentrations of brucine,strychnine,and the mixture of brucine and strychnine,total alkaloids from Strychni Semen for 24 h,the number of viable cells was observed using the MTT method. The cell apoptotic rate was detected and calculated by flow cytometry. Cytotoxicity on Ha Ca T cell was compared among alkaloids from Strychni Semen. RESULTS The proliferation rates and the apoptotic rate of strychnine on Ha Ca T were greater than brucine at the same concentration,the mixture of brucine and strychnine on Ha Ca T were greater than total alkaloids from Strychni Semen similarly in those two rates. CONCLUSIN The components of alkaloids from Strychni Semen have obvious cytotoxicity to Ha Ca T cell in a dose-dependent relationship,meanwhile,cytotoxicity of brucine is lower than that of strychnine.

关 键 词：人永生化表皮细胞 细胞毒性 马钱子总生物碱 马钱子碱 士的宁 

分 类 号：R966[医药卫生—药理学]